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  • Electronic Resource  (2)
  • calcium  (1)
  • methylxanthine  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Multiple effects of ethanol and 5-hydroxytryptamine on the gill cilia of mytilus edulis (1982)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 2 (1982), S. 215-224 
    Details
    ISSN: 0886-1544
    Keywords: Mytilus edulis ; 5-hydroxytryptamine ; cilia ; ethanol ; axoneme ; calcium ; filter feeding ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The lateral (L) cilia on an isolated filament from the gill of Mytilus edulis remain arrested at the end of their recovery stroke (hands up) when perfused with artificial sea water (ASW). The laterofrontal (LF) cilia continue to be active. The addition of 10% ethanol (ETOH) to the ASW perfusate arrests the LF cilia in a hands-up posture; the L cilia remain undisturbed. By contrast, 10-6 M 5-hydroxytryptamine (5HT) in ASW activates the L cilia and arrests the LF cilia at the end of their effective stroke (hands down). Continued perfusion with 10% ETOH (v/v) in ASW/5HT restores activity to the LF cilia but arrests the L cilia (hands up). These effects are reversible and independent of external Ca2+.Following the detergent extraction of the filament, all gill cilia are inactive. The addition of 0.2 mM ATP in the presence of low Ca2+ (〈 10-7 M) reactivates all model cilia. Under these conditions, 5HT can no longer inhibit the activity of LF cilia and is not required for the activation of the L cilia. This suggests that 5HT acts at a membrane level. An increase in free Ca2+ (〉 10-6 M) arrests the L cilia hands up; the LF cilia remain active. Further Ca2+ increase (〉 10-3 M) induces hands-up arrest of the LF cilia, confirming that the Ca2+ threshold of the two ciliary types is different by several orders of magnitude.The addition of 10% ETOH in low Ca2+ to demembranated reactivated cilia arrests the L cilia hands up while the LF cilia continue to beat. Ten percent ETOH appears to interact with the axoneme, mimicking the effect of high Ca2+ and with the membrane to increase Ca2+ permeability and possibly to inactivate 5HT receptors. These results are discussed in terms of axonemal switching mechanisms and the physiological control of filter feeding in the lamellibranch gill.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970020303
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The antagonistic effects of 5-hydroxytryptamine and methylxanthine on the gill cilia of mytilus edulis (1985)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 5 (1985), S. 293-309 
    Details
    ISSN: 0886-1544
    Keywords: Mytilus edulis ; 5-hydroxytryptamine ; lateral cilia ; laterofrontal cirri ; beat frequency ; methylxanthine ; filter-feeding ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The laterofrontal (LF) cirri on isolated gill filaments of Mytilus edulis, prepared in natural seawater, are active and initially beat with an average frequency of about 8 Hz (with a range of 6-14 Hz). However, the lateral (L) cilia on these filaments are arrested in a position at the end of their recovery stroke. Perfusion of the filament with artificial seawater (ASW), with or without 1% ethanol, has little or no biological effect on the activity of the LF cirri, although a transitory decrease in frequency often accompanies the perfusion process. The L cilia remain arrested during perfusion with ASW. The exposure of the gill to low levels of 5-hydroxytryptamine (5HT) (10-8 〈 5HT 〈 10-7 M) has no effect on the activity of the LF cirri but stimulates the L cilia to beat. Exposure to higher concentrations of 5HT (〉 10-7 M) elevates the beat frequency of the L cilia and simultaneously inhibits the activity of the LF cirri, leading to their arrest in a position at the end of the effective stroke. This arrest of the LF cirri occurs as the L cilia attain a 5HT-induced beat frequency between 12 to 14 Hz. The influence of 5HT on the L cilia and the LF cirri can be reversibly mimicked or enhanced by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX). A concentration of 0.5 mM IBMX mimics low 5HT concentrations (about 10-7 M) by stimulating the L cilia to beat without affecting the beat frequency of the LF cirri. A combimation of 10-7 M 5HT and 0.5 mM IBMX in ASW mimics high (〉 10-6 M) 5HT concentrations by arresting the LF cirri and increasing the beat frequency of the L cilia. Under these conditions, the threshold of the LF cirri arrest response is again found to occur as the L cilia attain a beat frequency of 12 - 14Hz. These results suggest that the mechanisms of LF cirri arrest and L cilia activation are mediated by 5HT -induced changes in intracellular cyclic AMP levels.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970050403
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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