ISSN:
1573-5044
Keywords:
Catharanthus roseus
;
cell culture
;
phosphate
;
tryptophan decarboxylase
;
phenylalanine ammonia-lyase
;
secondary metabolites
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In cell suspension cultures of Catharanthus roseus a rapid accumulation of secondary compounds (tryptamine, indole alkaloids, phenolics) was observed after transfer of the cells into special ‘induction’-media devoid of phosphate and other essential growth factors [11, 14]. The increase of product levels was suppressed in the presence of phosphate which was almost completely taken up from the medium and accumulated by the cells within 48 h after inoculation. The activities of tryptophan decarboxylase (TDC), the first enzyme in indole alkaloid biosynthesis, and of phenyl-alanine ammonia-lyase (PAL), the key enzyme of phenylpropanoid biosynthesis, were influenced differently by phosphate. Whereas the accumulation of phenolics and PAL activity were similarly inhibited by low concentration of phosphate, the medium-induced enhanced activity of TDC was not affected although the product pools were considerably reduced. Some consequences for the regulation of secondary metabolism will be discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00039880
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