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  • Electronic Resource  (2)
  • siderophore  (1)
  • siderophores  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Synthesis of optically pure chrysobactin and immunoassay development (1996)
    Springer
    BioMetals 9 (1996), S. 377-383 
    Details
    ISSN: 1572-8773
    Keywords: chrysobactin ; Erwinia chrysanthemi ; immunoassay ; siderophore ; synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Chrysobactin (α-N-(2,3-dihydroxybenzoyl)-d-lysyl-l-serine), a siderophore that is essential for systemic virulence by plant pathogenic Erwinia chrysanthemi, was synthesized with high diastereomeric purity. Chrysobactin was prepared by coupling the N-hydroxysuccinimide ester of α-N-(2,3-dibenzyloxybenzoyl)-ε-N-Cbz-d-lysine with l-serine benzyl ester followed by deprotection via hydrogenolysis. Optically pure chrysobactin was obtained with 98% overall yield. A monoclonal antibody to ferric chrysobactin was developed and characterized as IgM. The antibody reacts with chrysobactin, ferric chrysobactin and less strongly with ferric dihydroxybenzoic acid. The antibody reacts weakly with the siderophores ferrichrome, A, ferric pseudobactin and ferric rhodotorulic acid. This antibody was used in a competitive immunoassay to detect ferric chrysobactin at 10−8 to 10−10 mol. This immunoassay may provide a useful method for the detection of chrysobactin in plant samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00140607
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Rhizosphere interactions and siderophores (1990)
    Springer
    Plant and soil 129 (1990), S. 101-107 
    Details
    ISSN: 1573-5036
    Keywords: biocontrol ; Gaeumannomyces ; iron ; monoclonal antibodies ; Pseudomonas ; siderophores
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Certain plant growth-promoting pseudomonads inhibit deleterious and pathogenic rhizosphere bacteria and fungi by producing siderophores. Properties of a siderophore transport system which might provide a competitive advantage under iron stress conditions include ability to utilize other organisms' siderophores, higher Fe(III) stability constant, faster kinetics of dissolution of Fe(III) minerals, more efficient transport system, and resistance to degradation. In order to determine the concentration and localization of siderophores in the rhizosphere monoclonal antibodies (Mabs) to ferric pseudobactin, the siderophore of Pseudomonas putida B10, have been developed. Several Mabs cross reacted differently with various pseudobactins. A growth medium has been developed for the study for siderophore-mediated rhizosphere interactions in the laboratory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00011696
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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