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Interaction of the CRP-cAMP complex with the cea regulatory region (1989)

Salles, Bernard ; Weinstock, George M.

Springer

Molecular genetics and genomics 215 (1989), S. 537-542

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ISSN: 1617-4623

Keywords: Catabolite repression ; SOS response ; Colicin E1 ; LexA protein ; Cyclic AMP

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Analysis of the induction of expression of cea-lacZ fusions in cya and crp mutants showed that catabolite repression affects the kinetics of induction and the rate of induced synthesis. In a cya mutant, addition of cAMP reduced the induction lag and increased the amount of β-galactosidase produced. The CRP-cAMP complex was found to bind to two sites 5′ to the cea promoter, but deletion analysis showed that only one of these was involved in the control of cea. Deletion of this site resulted in a loss of the stimulatory effects of cAMP in a cya mutant.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00427053

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