ISSN:
1615-6102
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A method is described for the construction and use of a non-polarizable microelectrode which is protected within a quartz “electrode chamber”. Thereby the electrode may be saturated with hydrogen or any other gas or liquid. Using it as a hydrogen electrode, potentiometric measurements were made in the protoplasm and cell-sap ofNitella. The protoplasm and cell-sap behaved very differently toward the hydrogen electrode. The protoplasm at once produced a potential of between + ·093 and + ·030 volts with respect to hydrogen zero. These values which obviously cannot represent the concentration of hydrogen ions within the protoplasm, are interpreted as indicating rather its oxidation-reduction potential. Potentiometric readings for the cell-sap gave pH values of 5·47 to 6·16. The latter value is regarded as more reliable.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02057000
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