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THE SYNTHESIS OF IMMUNOREACTIVE PROLACTIN BY DECIDUA-CHORION (1979)

Healy, D. L. ; Kimpton, W. G. ; Muller, H. K. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

BJOG 86 (1979), S. 0

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ISSN: 1471-0528

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Normal term human decidua-chorion, amnion and placenta were examined for possible synthesis of prolactin (PRL) by organ culture of tissue fragments in medium M199. Before incubation, higher PRL concentrations were found in decidua-chorion (27.7±5.7 ng/mg protein; mean±SE) and amnion (19.7±6.9 ng/mg protein) than in placenta (3.1±0.5 ng/mg protein; p 〈0.005). Incubated deciduachorion released significantly more PRL into culture medium than did amnion or placenta. After 72 hours culture, the PRL concentration of decidua-chorion remained high (17.9±6.4 ng/mg protein); that in amnion (2.3±1.9 ng/mg protein) fell to approach that of placenta (1.4±1.0 ng/mg protein). In further studies, fragments of decidua-chorion were incubated in medium RPMI 1640 without or with puromycin (0.04 mM) or cycloheximide (0.1 mM). A decrease in medium PRL concentration was observed within four hours of adding these inhibitors of protein synthesis. At four hours, the PRL content of decidua-chorion cultured in puromycin (12.4±3 8 ng/mg protein) or cycloheximide (11.9±2.9 ng/mg protein) was lower than tissue incubated in medium alone (25.5±4.1 ng/mg protein; p 〈0.05). Comparable differences in tissue content and medium concentration were also seen at 24 and 72 hours. We conclude that human decidua-chorion synthesizes a protein immunologically simjlar to PRL and that this tissue may represent the principal source of amniotic fluid PRL.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1471-0528.1979.tb11261.x

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Weissenberg Effect in the Thick White of the Hen's Egg (1961)

MULLER, H. G.

[s.l.] : Nature Publishing Group

Nature 189 (1961), S. 213-214

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Its theory is not clear and has been discussed by Garner and Nissan3, Rivlin4, Reiner et al.* and Roberts5. The effect occurs only rarely and has been observed in highly viscous solutions of rubber in benzene8, certain soaps in hydrocarbon solvents3, over-aged sweetened condensed milk2 and certain ...