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  • 1960-1964  (7)
  • 1850-1859
  • 1963  (7)
  • Biochemistry and Biotechnology  (7)
  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 221-230 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A process has been developed giving over-all yields of about 50%: which could be increased to about 80% with higher material costs and processing time. The preferred method of recovering nucleotides from the cytoplasm has been thermal extraction by means of a heat exchanger followed by filtration, protein precipitation, and carbon adsorption. The concentrated eluate from the carbon is absorbed onto Dowex 1 ion-exchange resin, and groups of nucleotides are removed by pH-defined eluents and recovered by a second carbon adsorption. Finally, standard ion-exchange resolutions have been used to recover individual nucleotides. Other biochemicals in the extract have been investigated, and preliminary work has been done on the combination of nucleotide extraction with the recovery of nucleic acids from the residual cells.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 309-330 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Biological purification of water pollutants depends upon a complex balance of organisms and environment conducive to effective metabolism. The bench scale test is useful to determine effects of a given waste water on that balance and to suggest controls promoting optimum purification. Applicability of laboratory results depends, in part, upon an experimental design similating anticipated operating conditions. This paper discusses advantages and limitations of historical bench-tests as a guide for design of tests giving data that are more useful during full-scale operation.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A 50-gal. growth chamber is described together with its air filter and humidifier. The unit was designed especially for the production of Neurospora crassa, and was found to be effective in producing bulk amounts of mycelia with a high specific activity of tryptophan synthetase.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 41-48 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Bacillus subtilis NRRL B-2612, isolated at this laboratory, was used to produce polyglutamic acid from devitalized wheat gluten. Conditions for production of the polypeptide were investigated. When NRRL B-2612 was grown on wheat gluten in shaken flasks with phsophate buffer and salts at pH 6.5, yields of polymer reflected a 25-40% incorporation of glutamic acid into polyglutamic acid. Repeated alcoholic precipitation yielded peptides containing between 45 and 90% D-glutamic acid. Physical and chemical properties of a typical purified polyglutamic acid indicated that the product was a gamma-linked polypeptide composed of 75% D-glutamic acid and 25% L-glutamic acid with a molecular weight of approximately 200,000.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 255-273 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Stabilization ponds have been widely accepted in recent years, following studies that established deeper understanding of the phenomena associated with the stabilization process. A compilation of information on the use of both sewage and industrial ponds is presented showing types and geographical location in the United States. A total of 1,304 stabilization ponds currently are used as the principal method of sewage treatment serving a population of 2,138,085. Ponds are used for tertiary treatment serving a population of 2,146,951, in 343 instances. Use of 827 industrial ponds by 31 industries in 44 States is also indicated. Indiana ranks first in usage with 159 ponds and Callfornia second with 140. Ponds were classed as effective by 66 percent of the 455 users who reported on this item. Of 149 reports on odors, 73% indicated nuisances. Insect problems were reported in only a small number of instances. Data are presented on design considerations and the mechanism of waste stabilization ponds. Aerobic ponds are given greatest emphasis. Photosynthesis and its dependence upon the algal mass, suitable temperature, incident light penetration, nutrient supply, and induced vertical mixing by wind are of prime importance in the stabilization mechanism. Odors are associated with prolonged anaerobic conditions, and these may persist up to 4 weeks following extended ice cover in cold climates, if BOD loadings are 25 lbs. per acre per day or greater. Nitrogen and carbon may be limiting factors in the development of an algal mass. Coliform, BOD, and nutrient removals are presented for various physical, chemical, and climatic conditions. Although fecal streptococci removal averaged 97% in a study pond, they were still discharged in the effluent at a rate of 700,000 per second in winter and 70,000 per second in summer. A striking similarity exists generally among the algal speciation in stabilization ponds, regardless of geographic location. The algal mass is, however, dependent upon unique pond conditions and location, and may vary upwards to nearly 5 million algal cells per milliliter, 34,000 p.p.m. by volume, or 30-35 tons per acre per year. The problem of blue-green algal mats and resultant pigpen odors in extremely localized areas is duscussed. Midges and midge larvae may have an impact on the nutrient cycle in a stabilization pond, and may pose a potential localized nuisance in some areas. Mosquito problems have usually been associated with growth of aquatic vegetation. Suggestions for minimizing the potential problem are discussed.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 275-285 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Phenol-bearing waste waters continue to be an area of wide concern for various industries faced with the task of cleaning up effluents containing relatively small amounts of these materials. The microbiological aspects of the degradation of these materials in pure culture and waste treatment plants is reviewed. Actual operating experiences at three plants of The Dow Chemical Company, where phenols are present in combination with other widely varying waste constituents, are discussed.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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