ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
It was established that phenol-2,4-disulfonyl chloride and α-naphthol-2, 4-disulfonyl chloride introduce new intramolecular or tertiary bonds into lysozyme. The phenolic hydroxyl group seems to make the cross linking reagents soluble in aqueous media; it was established that a sulfonylating; species does dissolve prior to hydrolysis but that the chlorosulfonyl groups are hydrolyzed within seconds of dissolution. New covalently linked residues were shown to be introduced by different, spectra. Peptide maps of the lysozyme treated with phenol-2,4-disulfonyl chloride. peroxidized, and digested with trypsin showed definite deletions and additions when compared with the control. Partial analysis of the peptides showed that cross linking between ε-amino groups of lysine residues had occurred. A certain degree of stabilization of lysozyme to inactivation by certain inactivating conditions was noted after treatment with the two reagents. Ultra-centrifugal analysis of the lysozyme treated with phenol-2,4-disulfonyl chloride showed that there was no detectable fraction having a higher molecular weight than native lysozyme. Tentative assignments of positions of cross linking were made.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.1964.360020406
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