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Synthesis of Antibody in vitro by Lymph Node Fragments using Absorbable Gelatin Sponges (1966)

RICHTER, M. ; JUHASZ, A. ; DRECHSLER, B. ; [et al.]

[s.l.] : Nature Publishing Group

Nature 210 (1966), S. 645-645

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Young, adult New Zealand rabbits were immunized with rat serum albumin (RSA) (prepared by ammonium sulphate fractionation of whole rat serum, pure by the criterion of immunoelectrophoresis) and bovine serum albumin (BSA) (obtained from Pentex Incorporated, Kankakee, Illinois). The rabbits were ...

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1038/210645a0

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Photometrische Titration der Stärkepolysaccharide mit Jod (1966)

Richter, M.

Springer

Fresenius' Zeitschrift für analytische Chemie 222 (1966), S. 381-388

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ISSN: 1618-2650

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Description / Table of Contents: Zusammenfassung Die quantitative Bestimmung der beiden Stärkekomponenten beruht auf der Beobachtung, da\ die Jodsorption von Amylose schon bei einer Konzentration an freiem Jod [Jf] von etwa 5 · 10−5 n beendet ist, während das Amylopektin erst bei höheren [Jf]-Werten merkliche Mengen davon binden kann. Der genaue Endpunkt der Jodaufnahme von Amylose wird durch photometrische Titration bei 625 nm mit 0,005 n Jodlösung in An- und Abwesenheit einer bekannten Amylosemenge bestimmt. Aus dem Unterschied der Extinktionen vor Beginn der Reaktion des Amylopektins mit Jod wird die Amylosekonzentration des untersuchten Präparates berechnet. Für die Analyse werden je nach dem Amylosegehalt nicht mehr als 1–10 mg Probe/100 ml benötigt. Fehler der Extinktionsbestimmung: ±2–3%.

Notes: Summary The quantitative determination of starch components is based on the observation that the uptake of iodine by amylose is complete at about 5 · 10−5 n free iodine concentration (under the conditions of the present investigations) while the amylopectin can bind iodine only at a higher free iodine concentration. The exact end-point of the iodine uptake by amylose has been determined by photometric titration at 625 nm with 0.005 n iodine solution before and after adding of a known amount of amylose to the investigated starch solution. The original amylose content has been calculated on the basis of the difference between the extinction values measured in absence and in presence of the extra-added amylose. The extinction values were determined before the beginning of the amylopectiniodine reaction. For such a determination a 1–10 mg sample is sufficient; the error of the extinction measurement is not higher than ±2–3%.