Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Journal of neurochemistry
18 (1971), S. 0
ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract— Particle-bound hexokinase of rat brain homogenates was solubilized by successive treatment with 0-9 M-NaCl or 003 M-ATP (pH 8.0) and 0-5% (w/v) Triton X-100. This solubilized hexokinase and the soluble hexokinase present in cytoplasm of rat brain homogenates were chromatographed on DEAE-cellulose and some kinetic properties of the isolated hexokinase peaks were studied. The chromatographic separation was greatly influenced by the EDTA-concentration of the buffer used. No significant differences were observed in the chromatographic pattern and in the apparent Km-values for ATP and glucose and the apparent Kt for glucose-6-phosphate (versus ATP) between the soluble and particulate hexokinase solubilized by different reagents. On agarose-electrophoresis the solubilized particulate enzyme migrates as one single band, the soluble hexokinase separates into one major and two minor bands.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1971.tb12002.x
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