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  • 1970-1974  (3)
  • 1974  (3)
Material
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  • 1970-1974  (3)
Year
  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 249 (1974), S. 279-280 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In a later series of experiments involving further characterisation of the GPT isozymes in the hybrids and their parental cells, we have found strong evidence that the screening procedures used do not score different enzymes (GPT and LDH) but the same gene product (LDH) by different methods. The ...
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Human genetics 〈Berlin〉 22 (1974), S. 75-80 
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Eine einfache, rasche und direkte Färbemethode für die Darstellung der Hypoxanthin-Guanin-Phosphoribosyltransferase wird beschrieben. Sie beruht auf der Umwandlung von Inosin-Monophosphat in Hypoxanthin, das dann enzymatisch oxydiert wird. Diese Oxydation ist mit einer Reduktion eines Tetrazoliumsalzes zu blauem Formazan gekoppelt. Die Elektrophorese von HGPRT in Mensch-Chinesischer Hamster-Hybridzellen zeigt ein Muster mit drei Banden, das eine dimere Struktur dieses Enzyms nahelegt.
    Notes: Summary A simple, fast and direct staining method for the detection of hypoxanthineguanine phosphoribosyltransferase is described. It is based on the conversion of inosine monophosphate to hypoxanthine, which is then enzymatically oxidized. This oxidation is coupled to the reduction of a tetrazolium salt to blue formazan. The electrophoretic pattern of HGPRT in man-Chinese hamster hybrid cells shows a three-banded pattern, suggesting a dimeric structure for this enzyme.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Techniques are described for the electrophoretic characterization on Cellogel of adenosine deaminase, adenylate kinase, enolase, fumarase, α-galactosidase, glucosephosphate isomerase, glutathione reductase, glutamic-oxaloacetic transaminase, guanylate kinase, hexosaminidase, hypoxanthine-guanine phosphoribosyltransferase, indophenol oxidase, “malic” enzyme, mannosephosphate isomerase, nucleoside phosphorylase, peptidase-A, peptidase-B, peptidase-C, phosphoglucomutase, pyruvate kinase, triosephosphate isomerase, and UDP glucose pyrophosphorylase1 in the man-Chinese hamster somatic cell hybrid systems.
    Type of Medium: Electronic Resource
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