ISSN:
1432-2013
Keywords:
Insulin release
;
Pancreatic β-cells
;
β-Cells in suspension
;
Intact pancreatic islets
;
Glucose oxidation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The overall dynamics of glucose-induced insulin release was strikingly similar in dispersed cells and intact islets perifused in parallel. Both preparations exhibited a latency of 1–2 min, after which period there was a brisk rise of insulin release followed by a sustained second phase. During the second phase, insulin release from dispersed cells attained a stable plateau rate, whereas the release from intact islets continued to rise. Epinephrine (1 μM) inhibited the release in both preparations, but the return to basal rate was faster in the dispersed cells than in the intact islets. The dispersed cells oxidized glucose at a constant rate for at least 60 min; the glucose oxidation was markedly sensitive to changes of the glucose concentration in the range of 3–20 mM.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00585876
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