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  • 1985-1989  (1)
  • 1975-1979  (2)
  • 1988  (1)
  • 1978  (2)
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  • 1985-1989  (1)
  • 1975-1979  (2)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Light microscopic triple-colored immunohistochemical staining on the same vibratome section using the avidin-biotin-peroxidase complex technique (1988)
    Springer
    Histochemistry and cell biology 88 (1988), S. 353-356 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The sequential application of the avidin-biotinperoxidase complex technique was used to localize multiple tissue antigens on a single free floating section of rat brain. sequential visualization of individual antigens was achieved by the silver-gold-intensified diaminobenzidine (DAB) in the first step, nickel-intensified DAB in the second step, and the DAB alone in the third step of the immunostain procedure. For the demonstration of this method, tyrosine hydroxylase (TH), corticotropin-releasing factor (CRF), and vasopressin (VAS) antisera were used. Sections from the hypothalamic paraventricular nucleus (PVN) of rats pretreated with colchicine were stained. Black TH containing cell bodies were clearly distinguished from blue stained CRF cells and from yellow stained VAS-containing cell bodies in the PVN on the 25–30 μm thick vibratome sections. The sequential immunostaining procedure presented here results in superior staining of multiple antigens as compared to that achieved by the sequential application of the peroxidase-antiperoxidase (PAP) technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00570294
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Thermal polycondensation of ammonium paratungstate, (NH4)10[W12O40(OH)2].4H2O (1978)
    Springer
    Journal of materials science 13 (1978), S. 2541-2547 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Connected with the examination of the thermal polycondensation of ammonium paratungstate pentahydrate the chemical and morphological properties of intermediate phases formed during the thermal decomposition of APT have been investigated. We have studied the pH and the turbidity of the aqueous solutions of the intermediate phases, the solubility of the phases, and their rehydratation capability as well as the morphology of the crystallite granules and the grain size distribution. These properties of the original APT have been related to the same properties of the products of decomposition formed between different temperature ranges. The results obtained show unambiguously that each of the above mentioned properties suddenly changes in the temperature range 225 to 250°C. This temperature range coincides with the formation temperature of a new phase called APT II. The most probable formula of APT II is (NH4)8 [H2W13O43(OH)2]·H2O.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02402739
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Thermal polycondensation of ammonium paratungstate, (NH4)10[W12O40(OH)2].4H2O (1978)
    Springer
    Journal of materials science 13 (1978), S. 2541-2547 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Connected with the examination of the thermal polycondensation of ammonium paratungstate pentahydrate the chemical and morphological properties of intermediate phases formed during the thermal decomposition of APT have been investigated. We have studied the pH and the turbidity of the aqueous solutions of the intermediate phases, the solubility of the phases, and their rehydratation capability as well as the morphology of the crystallite granules and the grain size distribution. These properties of the original APT have been related to the same properties of the products of decomposition formed between different temperature ranges. The results obtained show unambiguously that each of the above mentioned properties suddenly changes in the temperature range 225 to 250°C. This temperature range coincides with the formation temperature of a new phase called APT II. The most probable formula of APT II is (NH4)8 [H2W13O43(OH)2]·H2O.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552682
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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