ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
An improved semiautomated enzymatic procedure employing L-ly-sine decarboxylase for the analysis of lysine is described. Carbon dioxide formed in the reaction mixture is separated by a gas dialyzel and measured calorimetrically by the phenolphthalein method. Certain vitamins, amino acids, and metal ions, when added to lysine standard solution and analyzed by the present procedure, caused a decrease in both sensitivity and reproducibility. The procedure developed is capable of analyzing 30 samples per hour. The mean recovery of added lysine was 94.1%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1979.tb03816.x
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