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  • 1980-1984  (9)
  • 1982  (9)
  • Chemistry  (6)
  • Life and Medical Sciences  (2)
  • benzo(a)pyrene  (1)
  • cyst(e)ine determination  (1)
Source
Material
Years
  • 1980-1984  (9)
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Absorption and fluorescence spectra ofS-quinolylethylated Kunitz soybean trypsin inhibitor (1982)
    Springer
    The protein journal 1 (1982), S. 225-240 
    Details
    ISSN: 1573-4943
    Keywords: protein disulfide bonds ; reductive alkylation ; soybean trypsin inhibitor (Kunitz) ; UV absorption spectra ; fluorescence spectra ; S-β-2-quinolylethylation ; cyst(e)ine determination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Disulfide bonds in soybean trypsin inhibitor (Kunitz) were simultaneously reduced and alkylated using tri-n-butylphosphine and 2-vinylquinoline at pH 7.6 in 0.11 M Tris-4.4 M urea, 41% ethanol. The resulting S-β-2-quinolylethylated protein (2-QE-STI) has a new absorption peak at 315–318 nm. Its quinoline fluorescence can be excited above 310 nm independently of intrinsic protein fluorescence. Free 2-quinolylethylcysteine (2-QEC) shows unexpectedly weak fluorescence. Quinoline absorption in 2-QEC and 2-QE-STI changes with pH. The apparentpK values determined spectrophotometrically are near 5 for 2-QEC and 3 for 2-QE-STI. Fluorescence decreased with increasing pH and in the presence of chloride ions. Both structural and charge effects thus appear to influence the absorption and fluorescence of the quinoline group. Corrected fluorescence emission (excited at 316 nm) of neutral 2-QE-STI diluted in 0.1 N H2SO4 was directly proportional to concentration in the range 0.4–8 μm 2-QEC. The 2-QEC content of the protein derivative determined by UV absorption at pH 1.5 was in agreement with the expected value of four residues per mole. Fluorescence measurements ofS-2-quinolylethylated proteins may be especially useful as a sensitive, specific assay for cyst(e)ine residues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01025001
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  • 2
    Electronic Resource
    Electronic Resource
    Vapor-liquid equilibria for benzene/polybutadiene and cyclohexane/polybutadiene systems at 333, 355, and 373 K using gas chromatography (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 27 (1982), S. 3067-3077 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A perturbation chromatography apparatus has been designed and constructed for determining the vapor-liquid equilibrium between a two-component (solvent/helium) vapor phase and a two-component (polymer/solvent) liquid phase. The apparatus performed very well, giving reproducible and reliable results that agree with independent, previously reported studies. All tests of the equipment indicated that it was successful in meeting the conditions of low column pressure drop, small perturbations, and slow flow rate that are required for perturbation chromatography. Binary polymer/solvent data were obtained for polybutadiene (PBD)/benzene or polybutadiene/cyclohexane systems at solvent partial pressures to 40 kPa and for n-hexane at infinite dilution, all at the three temperatures of 333.15, 355.00, and 373.15 K. The experimental data for each system can be represented within experimental error by the Flory-Huggins polymer solution theory using a single binary interaction parameter that is independent of temperature and concentration.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1982.070270829
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  • 3
    Electronic Resource
    Electronic Resource
    Stabilization of glucose oxidase by immobilization/modification as a function of pH (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 1225-1231 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260240520
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  • 4
    Electronic Resource
    Electronic Resource
    Metabolism of Benzo(a)pyrene by Human Epithelial and Fibroblastic Cells: Metabolite Patterns and DNA Adduct Formation (1982)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 18 (1982), S. 135-148 
    Details
    ISSN: 0730-2312
    Keywords: DNA adduct formation ; benzo(a)pyrene metabolism ; human cells ; mammary fibroblasts ; mammary epithelial cells ; metabolite patterns ; benzo(a)pyrene ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: We demonstrate in cell culture that mammary epithelial cells from normal human breast specimens metabolize benzo(a)pyrene (BaP) and form adducts with the bases of their DNA more readily and at lower concentrations of BaP than do fibroblasts from the same specimens. BaP metabolism and adduct formation was determined in the same incubations with epithelial cells grown out in early passage from each of three specimens and with fibroblasts from one of these specimens. The metabolite pattern of the epithelial cells was indicative of preferential formation of 7, 8-dihydrodiol-9, 10-dihydroepoxybenzo(a)pyrene the ultimate carcinogen. In contrast, fibroblasts formed mainly mono- and dihydroxide derivatives of BaP. The metabolite pattern from epithelial cells was compatible with the ease in which adducts between DNA and the diolepoxide of benzo(a)pyrene were formed. These results provide evidence that chemical carcinogens should be considered as possible factors in the induction of breast cancer in women.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.1982.240180202
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  • 5
    Electronic Resource
    Electronic Resource
    Changes in Raman vibrational bands of calf thymus DNA during the B-to-A transition (1982)
    New York : Wiley-Blackwell
    Biopolymers 21 (1982), S. 499-512 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The B-to-A conformational transition of calf thymus DNA fibers was followed employing Raman spectroscopy. The transition was induced by soaking DNA fibers in water/ethanol mixtures increasing from 60 to 85% ethanol (v/v). Intensity changes of 17 Raman vibrational bands were quantified in the region from 400 to 860 cm-1. Two bands at 500 and 784 cm-1 were employed as internal standards. These bands do not appear to change in intensity with ethanol concentration. Large intensity changes relative to these two bands are observed between 70 and 74% ethanol for backbone vibrations at 708, 808, and 835 cm-1, and base vibrations at 682, 730, and 750 cm-1. These results indicate that a highly cooperative conformational change takes place between different portions of DNA in the B-to-A transition. Relative intensity changes preceding the onset of the major transition are observed in only two bands; at 835 cm-1, assigned to a ribose-phosphate vibration, and at 750 cm-1, assigned to thymine. The implications of these pretransition changes are discussed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360210303
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Effects of guanethidine-induced sympathectomy on morphology of the pre- and postclosure lleum of the neonatal rat (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 164 (1982), S. 175-186 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The disappearance of the characteristic supranuclear vacuole and extensive apical canalicular system from enterocytes of the ileal villi occurs during the third postnatal week in rats. This phenomenon is associated with loss of permeability of these cells to macromolecules and is therefore termed closure. The present study was designed to analyze the influence of neonatal guanethidine (GTN)-induced sympathectomy on the morphology of the pre- and postclosure ileum of the rat. Light and electron microscopy of control and GTN-sympathectomized rats demonstrated the retention of immature, vacuolated cells on ileal villi as late as 23 days postnatally in GTN-treated rats. Villi from control rats contained only adultlike nonpermeable cells. Electron microscopy further demonstrated no structural differences in the apical canalicular system or storage vacuoles of the delayed cells in GTN rats when compared to the ileal epithelium from preclosure time periods (7 and 15 days) in both GTN-sympathectomized and control rats. Goblet cells were counted on Periodic-Acid-Schiff-stained sections of ileum from 7, 15, and 23-day GTN and control rats. The percentage of goblet cells in the total epithelial cell population of the villus was significantly higher in control versus GTN rats at all time periods. The percentage of goblet cells increased in both groups from day 7 to 15. However, closure in the control group (approximately day 18) was coincident with a steep increase in the percentage of ileal goblet cells which was not evident in the goblet-cell population of the GTN villus. This pattern of change in control versus GTN goblet-cell production was correlated with a similar pattern of variation in the number of crypt cell mitoses between the two groups over the same time period.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001640207
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  • 7
    Electronic Resource
    Electronic Resource
    Electron diffraction study of pristine-and iodine-doped poly(cis-acetylene) (1982)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Letters Edition 20 (1982), S. 97-102 
    Details
    ISSN: 0360-6384
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1982.130200204
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  • 8
    Electronic Resource
    Electronic Resource
    Structure of iodine doped polyacetylene (1982)
    Basel : Wiley-Blackwell
    Die Makromolekulare Chemie, Rapid Communications 3 (1982), S. 269-274 
    Details
    ISSN: 0173-2803
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/marc.1982.030030503
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  • 9
    Electronic Resource
    Electronic Resource
    An estimate of bond length alternation in trans-polyacetylene (1982)
    Basel : Wiley-Blackwell
    Die Makromolekulare Chemie, Rapid Communications 3 (1982), S. 655-659 
    Details
    ISSN: 0173-2803
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/marc.1982.030031001
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