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  • 1
    Electronic Resource
    Electronic Resource
    Application of immunoblotting to serum protein phenotyping with reference to α2HS-glycoprotein (AHS) typing of bloodstains (1985)
    Springer
    International journal of legal medicine 95 (1985), S. 153-158 
    Details
    ISSN: 1437-1596
    Keywords: Serum groups, immunoblotting ; α2HS-glycoprotein typing ; Bloodstains, immunoblotting ; Serumgruppen, Immunoblottierung ; α2HS-Glykoprotein, Typisierung ; Blutspuren, Immunoblottierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Seren und Blutextrakte wurden auf Polyacrylamidgelen isoelektrisch fokussiert. Die fokussierten Proteine wurden auf Nitrozellulosemembranen durch Diffusion oder elektrophoretisch transferiert. Durch ein spezifisches Antiserum erfolgte auf den Membranen die AG-AK-Reaktion. Nach Waschen der Membranen wurde der gebundene Antikörper mit einem enzymisch markierten Anti-Kaninchen IgG-Antikörper nachgewiesen. Mit dieser Technik konnten die gruppenspezifische Komponente, das α2HS-Glykoprotein, die Komplementkomponenten C6 und C7, der Gerinnungsfaktor 13B und das Plasminogen sehr empfindlich phänotypisiert werden. Vorzugsweise konnten bis zu 6 Monate alte Blutspuren richtig typisiert werden.
    Notes: Summary Sera and bloodstain extracts were subjected to isoelectric focusing in polyacrylamide gels. The focused proteins were transferred to nitrocellulose membranes by diffusion or electrophoretically, then allowed to react with specific antiserum and, after washing, with peroxidase-labeled anti-rabbit IgG. The immune complexes formed on the membranes were detected with 4-chloro-1-naphthol and hydrogen peroxide. Serum group-specific component, α2HS-glycoprotein, the sixth and the seventh component of complement, factor 13B, and plasminogen could be phenotyped with high sensitivity. Bloodstains as old as 6 months could be correctly typed for α2HS-glycoprotein by the blotting technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201072
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cell proliferation and cell loss in intramucosal signet ring cell carcinoma of canine stomachs induced by N-ethyl-N′-nitro-N-nitrosoguanidine (1985)
    Springer
    Journal of cancer research and clinical oncology 110 (1985), S. 87-94 
    Details
    ISSN: 1432-1335
    Keywords: Stomach ; Signet ring cell carcinoma ; Cell turnover ; Tritiated thymidine autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Signet ring cell carcinoma was induced in canine stomachs by N-ethyl-N′-nitro-N-nitrosoguanidine, and modes of cell proliferation and turnover in the carcinoma were studied by 3H-thymidine autoradiography in conjunction with morphometric analysis. From 2 to 15 months after the cessation of 8 months carcinogen treatment, carcinomas in an early stage were obtained. Most of the cancer tissues confined to the lamina propria showed a layered structure. This comprised three layers; the superficial and the deep layer were composed of signet ring cells, and the middle layer was composed of small round cells. The dogs were labeled with 3H-thymidine by s.c. injection and by local infusion of the celiac artery. Flash-labeled autoradiographs revealed that most 3H-thymidine incorporating cancer cells were located around the middle layer, with a small amount of mucin. Using a pulse labeling experiment, those labeled carcinoma cells were shown to migrate from the middle layer towards the surface. Morphometric analysis of the autoradiographs showed that the small cells in the middle layer migrated upwards and produced mucin to become full-blown signet ring cells by 5.5 days. In 15 days, most labeled cancer cells in the superficial layer had disappeared. This mode of cellular turnover appeared to mimic a cell renewal system of the normal gastric mucosa. If the cancer cells turn over in this way, the tumor must grow slowly, remaining as an intramucosal cancer for a relatively long period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402717
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    Paper (German National Licenses)
    Fulltext
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