ISSN:
0935-6304
Keywords:
Gas chromatography, GC
;
Capillary columns
;
Mass spectrometry
;
Autoderivatization
;
Cyclophosphamide
;
4-Ketocyclophosphamide
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The selectivity of a capillary gas chromatographic assay for the anti-neoplastic and immunosuppressive agent cyclophosphamide (CPA) towards one of its naturally occurring metabolites, i.e. 4-ketocyclophosphamide (4-keto CPA), has been studied. Mass spectrometry studies showed that a cyclization product of 4-keto-CPA can form using the same chromatographic conditions as those under which CPA was determined. However, the amount produced is relatively small (less than 10%) compared with the percentage produced from CPA. Furthermore, both cyclization products, formed by loss of HCI and intraalkylation of the parent compounds, can be separated well under suitable chromatographic conditions.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240090207
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