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High Pressure Liquid Chromatography Determination of Histamine in Fish (1987)

GOUYGOU, J. P. ; SINQUIN, C. ; DURAND, P.

Oxford, UK : Blackwell Publishing Ltd

Journal of food science 52 (1987), S. 0

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ISSN: 1750-3841

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology

Notes: Histamine was determined by reversed-phase high-pressure liquid chromatography in trichloroacetic acid extracts after derivatization with o-phthaladehyde. Fluorescence was monitored at 350 nm excitation and 450 nm emission wavelength after elution with water containing 40% acetonitrile. The advantage of the described method is a rapid analysis in an automated system, where no selective extraction procedure is necessary and interfering substances are easily separated from the histamine fluorophore.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-2621.1987.tb14244.x

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