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  • 1990-1994  (2)
  • 1985-1989  (4)
  • 1992  (2)
  • 1988  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Contribution of neural intrapancreatic non-cholinergic non-adrenergic mechanisms to glucose-induced insulin release in the isolated rat pancreas (1992)
    Springer
    Diabetologia 35 (1992), S. 1133-1139 
    Details
    ISSN: 1432-0428
    Keywords: Intrinsic nerves ; isolated rat pancreas ; insulin ; somatostatin ; glucagon ; glucose ; adrenergic ; cholinergic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the isolated rat pancreas the effect of intrapancreatic non-adrenergic non-cholinergic nerves was examined upon insulin, glucagon and somatostatin release during perturbations of perfusate glucose. Elevation of glucose from 1.6 to 8.3 mmol/l increased insulin and somatostatin secretion and inhibited glucagon release. The first phase of insulin secretion was significantly reduced by the neurotoxin tetrodotoxin to 55% of the controls (p〈0.05). The somatostatin response was attenuated by tetrodotoxin while the change of glucagon remained unaffected. In contrast the combined adrenergic and cholinergic blockade with atropine, phentolamine and propranolol (10−5mol/l) did not modify the insulin, glucagon and somatostatin response. When glucose was changed from 8.3 to 1.6 mmol/l, the reduction of insulin and somatostatin release was not modified by tetrodotoxin, but stimulation of glucagon was significantly attenuated by 60–70% (p〈0.03), which was similar to the effect of combined adrenergic and cholinergic blockade. Subsequently, the effect of neural blockade was examined during more physiological perturbations of perfusate glucose levels. When glucose was changed from 3.9 to 7.2 mmol/l, tetrodotoxin also attenuated first phase insulin response by 40% while cholinergic and adrenergic blockade had no effect. The nitric oxide synthase inhibitor NG-Nitro-l-arginine-methylester (l-NAME) did not alter the glucose-induced insulin response indicating that nitric oxide is not involved in this mechanism. It is concluded that neural non-adrenergic noncholinergic mechanisms contribute to the first, but not second phase of glucose-induced insulin release. Non-adrenergic non-cholinergic effects do not participate in regulation of glucagon and somatostatin secretion under the conditions employed. The non-adrenergic non-cholinergic effect is most likely of peptidergic nature and remains to be examined in greater detail.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401366
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cross-coupled effects of repeated vestibular and optokinetic stimulations on the dynamics of the vestibulo-ocular and optokinetic reflexes in the cat (1988)
    Springer
    Experimental brain research 70 (1988), S. 209-215 
    Details
    ISSN: 1432-1106
    Keywords: Vestibuloocular reflex ; Optokinetic reflex ; Habituation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cats were submitted to repeated step stimulations either vestibular or optokinetic. Regardless of which of the two stimuli was used, dynamic modifications were observed in both vestibulo-ocular response and optokinetic after-nystagmus (OKAN). The progressive changes in post-rotational nystagmus and OKAN were quantified by measuring the duration of their primary phase. A parallel evolution of these two parameters was found. When repeated unidirectional vestibular stimulations were used, the same asymmetry was induced in both vestibuloocular responses and OKAN. These results support the hypothesis that the vestibulo-ocular and the optokinetic reflex share a common velocity storage mechanism, although alternative hypotheses cannot be excluded.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271861
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Plasma reaction of group VI metal carbonyls (1992)
    Springer
    Plasma chemistry and plasma processing 12 (1992), S. 147-159 
    Details
    ISSN: 1572-8986
    Keywords: Metal carbonyls ; PECVD ; chromium ; molybdenum ; tungsten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract The hexacarbonyl compounds of Cr, Mo, and W have been used as precursors in plasma-enhanced chemical vapor depositions (PECVD). They form films of good adherence on glass, ceramics, and a variety of polymers. The nature of the deposits depends very much on the composition of the gas, which forms the plasma. When pure argon is used, the resulting films contain considerable amounts of oxygen and carbon. Films deposited in hydrogen/argon mixtures consist of the metal and/or the carbide. With Ar/O2 mixtures, Mo(CO)6 and W(CO)6 are converted into films of MoO3 and WO3, respectively. When H2S/H2 mixtures are used as plasma gas, Mo(CO)6 yields films consisting of MoSx.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01447443
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Lake Torrens brine (1988)
    Springer
    Hydrobiologia 158 (1988), S. 267-269 
    Details
    ISSN: 1573-5117
    Keywords: Australia ; playa ; chloride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chloride content of the groundwater increases towards Lake Torrens. Major enrichment occurs on the playa and is caused by evaporation from the capillary zone. The chloride is sea derived via cyclic aeolian transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00026284
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Bestimmung des heißwasserlöslichen Borgehalts in Böden mit der induktionsgekoppelten Plasma-Atom-Emissionsspektrometrie (1988)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 331 (1988), S. 713-720 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The purpose of this study is to find out whether the previously applied procedures for determining hot water soluble boron trace contents in soils using dianthrimide or azomethin-H can be substituted by ICP-AES. Research was made into chemical and spectral interferences which can arise in all three methods. The 249.678 nm boron emission line is not affected by the sample matrix indicated. The boron content from more than 60 extracts was determined using the three methods mentioned and the results were compared by means of regression calculations. It was found that ICP-AES and the dianthrimide procedure do not differ significantly, whereas the azomethin-H technique leads to somewhat higher values. The accuracy of the results achieved by means of ICP-AES was confirmed by a collaborative study. In order to determine the concentration of boron in soils in the range of 0–2 mg/kg the ICP-AES can be applied following the hot water extraction without further pretreatment of samples. This method is superior in particular when additional elements are to be considered in the soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01105606
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Monosized, magnetic polymer particles: Their use in separation of cells and subcellular components, and in the study of lymphocyte function in vitro (1988)
    Chicester [u.a.] : Wiley-Blackwell
    Journal of Molecular Recognition 1 (1988), S. 9-18 
    Details
    ISSN: 0952-3499
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: By employing the principles of “activated swelling”, monosized, superparamagnetic polymer particles have been prepared ranging in size from 1-100 μm. Both during and after the swelling process, the particles can be modified to meet a series of specific demands making them potentially very interesting for many separation and assay purposes.Using monoclonal antibodies to direct the magnetic beads to their targets, immunomagnetic separation has turned out to be one of the most specific, reliable and, above all, the fastest technique available today to isolate particulate material for further studies. So far, most efforts have been concentrated on methodology for fractionation of cells in suspension, such as removal of tumour cells from bone marrow or isolation of lymphoid cells from peripheral blood. These studies have both established the parameters necessary for optimal performance and at the same time laid the groundwork for future developments making immunomagnetic separation an exciting new tool in many research areas.High speed and specificity are the most conspicuous features of immunomagnetic cell separation. These properties have been exploited in the successful development of a new technique for tissue typing of cells directly from peripheral blood specimens. Both higher sensitivity and specificity have been obtained. The same principles can be used for fast and safe quantification of cell populations and subpopulations in blood and cell suspensions.The functions of, and interactions between, peripheral blood cell populations or subpopulations in the immune response have also been studied with high precision. The significance of direct cell contact on the one hand, and soluble factors on the other, can now be established in detail. Immunomagnetic beads have also been used to study the interaction between various T lymphocyte membrane molecules in the early phases of the activation process.Finally, the usefulness of specially developed particles for the fractionation of subcellular components is described.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmr.300010104
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    Paper (German National Licenses)
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