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Identification and germline transformation of the ribosomal protein rp21 gene of Drosophila: Complementation analysis with the Minute QIII locus reveals nonidentity (1988)

Kay, Mark A. ; Zhang, J. -Y. ; Jacobs-Lorena, Marcelo

Springer

Molecular genetics and genomics 213 (1988), S. 354-358

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ISSN: 1617-4623

Keywords: Drosophila ; Minute ; Ribosomal protein ; Transformation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Minute loci represent a class of about 50 different Drosophila genes that appear to be functionally related. These genes may code for components of the protein synthetic apparatus. While one Minute locus has been recently shown to code for a ribosomal protein, it is not yet known whether any of the other Minute loci also code for ribosomal proteins. We have addressed this question by a combined molecular and genetic approach. In this report, a cloned DNA encoding the ribosomal protein rp21 is partially characterized. The rp21 gene maps to the same region (region 80 of chromosome 3L) as the temperature-sensitive Minute QIII gene. Using P-element mediated transformation, the rp21 gene was transformed into the germline of Drosophila. RNA blot experiments revealed that the transformed gene is expressed in transgenic flies. However, genetic complementation analysis indicated that the QIII locus and the rp21 gene are not identical. Implications of these findings for the relationship between Minutes and ribosomal protein genes are discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00339602

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High-resolution electron microscopy of high-temperature superconductors (1988)

Marks, L. D. ; Li, D. J. ; Shibahara, H. ; [et al.]

New York, NY : Wiley-Blackwell

Journal of Electron Microscopy Technique 8 (1988), S. 297-306

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ISSN: 0741-0581

Keywords: Defects ; Polymorphs ; Structure ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Natural Sciences in General

Notes: We review recent results obtained at Northwestern using high-resolution electron microscopy to study high-temperature superconductors. While in general these materials form large, very perfect single crystal grains which display very few imperfections, there is also evidence of slip defects, amorphous regions, and order-disorder transformations. We also report that the gadolinium-based superconductors and in one case yttrium-based superconductors show evidence for some copper solid solubility in the form of copperrich planar defects. The structure of a metastable trigonal polytype is also reported, as are the effects of electron beam and water vapor damage to the materials.

Additional Material: 9 Ill.