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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of the 5’Region of the Human Tyrosine Hydroxylase Gene: Combinatorial Patterns of Exon Splicing Generate Multiple Regulated Tyrosine Hydroxylase Isoforms (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 50 (1988), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A single human gene has been described to encode multiple tyrosine hydroxylase (TH) mRNAs. The study of this variation has been extended by S1 mapping experiments and by analysis of the 5’region of the TH gene. Four different mRNAs were found to originate solely from alternative splicing of two exons. Comparison of the 5’flanking regions of human and rat genes discloses several highly conserved segments, likely to play an important role in the regulation of TH gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1988.tb03009.x
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  • 2
    Electronic Resource
    Electronic Resource
    Preparation of β-Lactoglobulin and p-Lactoglobulin-Free Proteins from Whey Retentate by NaCI Salting Out at Low pH (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 53 (1988), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Solubility of the main proteins in 10 x acid and rennet whey retentates was studied in the pH range 2.0 to 4.0 in the presence of NaCI from 2 to 15% (w/v) final concentration, at 20°C to find fractionation conditions suitable for preparing pure β-lactoglobulin and β-lactoglobu-lin-free whey proteins and scaling up. At pH 2.0, 7% NaCI, 20 min holding time, nearly all (3-lactoglobulin remained soluble while a precipitate (PI) containing all other proteins was formed. Pure p-lacto-globulin was quantitatively recovered by salting-out the centrifugation supernatant at 30% NaCI (w/v) final concentration. PI, insoluble at pHs lower than 4.0, was made soluble at any pH by dissolving at pH 9.0, dialyzing against 50 mM formic acid (pH 3.0) and freeze-drying.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1988.tb08945.x
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  • 3
    Electronic Resource
    Electronic Resource
    Total synthesis of peramine (1988)
    s.l. : American Chemical Society
    The @journal of organic chemistry 53 (1988), S. 4650-4653 
    Details
    ISSN: 1520-6904
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jo00255a002
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  • 4
    Electronic Resource
    Electronic Resource
    Attempts to transform Zea mays via pollen grains (1988)
    Springer
    Sexual plant reproduction 1 (1988), S. 156-163 
    Details
    ISSN: 1432-2145
    Keywords: Corn ; Transformation ; Pollen and stigma nucleases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An attempt was made to transfer two sorts of DNA into maize via pollen grains. Controls for both pollen quality and DNA behaviour during the transformation experiments were included. When genomic DNA was used, no transformants were observed among the 1805 seeds screened. With plasmid DNA (harbouring the gene expressing kanamycin resistance in plant cells), three plants with kanamycin resistance were observed among the 1723 seeds screened, although no molecular evidence of transformation was obtained. Experiments indicated that under the conditions used, DNA was being degraded by both pollen and stigma nucleases. Consequently, we attempted to determine protocols which would inhibit these nuclease activities in order to preserve DNA integrity during transformation experiments, thus allowing fertilization. We found that a classic germination medium supplemented by 300 or 600 mM KNO3, or 20% PEG1550 satisfied all these conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193746
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  • 5
    Electronic Resource
    Electronic Resource
    Purification of tobacco O-methyltransferases by affinity chromatography and estimation of the rate of synthesis of the enzymes during hypersensitive reaction to virus infection (1988)
    Springer
    Planta 176 (1988), S. 36-41 
    Details
    ISSN: 1432-2048
    Keywords: Hypersensitivity ; Lignification ; O-methyltransferase ; Nicotiana (enzyme synthesis during TMV infection) ; Tobacco mosaic virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The three tobacco (Nicotiana tabacum L.) S-adenosyl-L-methionine: o-diphenol-O-methyltransferases (OMTs; EC 2.1.1.6) were purified to homogeneity by affinity chromatography on adenosine-agarose. Amounts and catalytic actities of the enzymes were measured in tobacco leaves during the hypersensitive reaction to tobacco mosaic virus. The drastic increase in activity of each enzyme upon infection was shown to arise from the accumulation of enzymatic protein with constant specific enzymatic activity. Rates of OMT synthesis were determined from pulse-labeling experiments with L-[14C]leucine injected into the leaves. The specific radioactivities of the homogenous enzymes were compared in healthy and tobacco mosaic virus-infected tobacco. The results demonstrated that increase in OMT amounts is a consequence of de novo synthesis of the enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392477
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  • 6
    Electronic Resource
    Electronic Resource
    MEDIT: an interactive graphics editor for molecular envelopes (1988)
    Copenhagen : International Union of Crystallography (IUCr)
    Applied crystallography online 21 (1988), S. 985-987 
    Details
    ISSN: 1600-5767
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: MEDIT is a computer program for the interactive editing or ab initio construction of molecular envelopes on the Evans & Sutherland PS300 graphics display. It has been designed as a tool for molecular replacement and electron density modification methods used in the determination of macromolecular crystal structures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S002188988800932X
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  • 7
    Electronic Resource
    Electronic Resource
    Restrained refinement of two crystalline forms of yeast aspartic acid and phenylalanine transfer RNA crystals (1988)
    [S.l.] : International Union of Crystallography (IUCr)
    Acta crystallographica 44 (1988), S. 112-124 
    Details
    ISSN: 1600-5724
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Four transfer RNA crystals, the monoclinic and orthorhombic forms of yeast tRNAPhe as well as forms A and B of yeast tRNAAsp, have been submitted to the same restrained least-squares refinement program and refined to an R factor well below 20% for about 4500 reflections between 10 and 3 Å. In yeast tRNAAsp crystals the molecules exist as dimers with base pairings of the anticodon (AC) triplets and labilization of the tertiary interaction between one invariant guanine of the dihydrouridine (D) loop and the invariant cytosine of the thymine (T) loop (G 19-C56). In yeast tRNAPhe crystals, the molecules exist as monomers with only weak intermolecular packing contacts between symmetry-related molecules. Despite this, the tertiary folds of the L-shaped tRNA structures are identical when allowance is made for base sequence changes between tRNAPhe and tRNAAsp. However, the relative mobilities of two regions are inverse in the two structures with the AC loop more mobile than the D loop in tRNAPhe and the D loop more mobile than the AC loop in tRNAAsp. In addition, the T loop becomes mobile in tRNAAsp. The present refinements were performed to exclude packing effects or refinement bias as possible sources of such differential dynamic behavior. It is concluded that the transfer of flexibility from the anticodon to the D- and T-loop region in tRNAAsp is not a crystalline artefact. Further, analysis of the four structures supports a mechanism for the flexibility transfer through base stacking in the AC loop and concomitant variations in twist angles between base pairs of the anticodon helix which propagate up to the D- and T-loop region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S010876738700446X
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  • 8
    Electronic Resource
    Electronic Resource
    Relapsing hepatitis in a child, associated with isolation of hepatitis A virus antigen from the liver (1988)
    Springer
    European journal of pediatrics 147 (1988), S. 333-333 
    Details
    ISSN: 1432-1076
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00442712
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  • 9
    Electronic Resource
    Electronic Resource
    Localization of adenylate cyclase activity in Populus: its relation to pollen-pistil recognition and incompatibility (1988)
    Springer
    Sexual plant reproduction 1 (1988), S. 140-149 
    Details
    ISSN: 1432-2145
    Keywords: Interspecific incompatibility ; Stigma-pollen interactions ; Adenylate cyclase ; Cytochemistry ; Populus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Adenylate cyclase has been localized cytochemically in female and male parents as well as during the pollen-stigma interaction with an original technique employing strontium as the capture ion and adenyl imidodiphosphate as the specific substrate. The specificity of the reaction was checked by using several controls. No final specific reaction product was detected in unpollinated P. deltoides stigmas or in the P. deltoides or P. alba pollen grains used for compatible and incompatible pollinations. In the compatible cross between P. deltoides × P. deltoides, fine dense precipitates were observed in the dictyosomes and the plasma membrane and exterior to the exine of hydrated pollen grains adhering to the stigma surface. Labeling of the stigmatic pellicle was also observed after pollen adhesion and hydration. This was accompanied by a strong reactivity of the cell wall and plasmalemma of the stigma papillae at the sites of pollen tube germination on the stigma surface and at the sites of penetration of pollen tubes between adjacent papillae. In the incompatible cross between P. deltoides x P. alba, adenylate cyclase activity was still present but reduced at the stigma surface following adhesion, hydration, and germination of P. alba pollen. This activity was completely abolished after the penetration of pollen tubes between stigma papillae. These findings suggest that in Populus, adenylate cyclase activity is correlated to pollen adhesion, hydration, and germination at the stigma surface, and that the abolition of this enzyme activity could be one of the cellular events governing the gametophytic phenotype of incompatibility in the cross between P. deltoides and P. alba.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193744
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  • 10
    Electronic Resource
    Electronic Resource
    New aspects of the polymorphism of cyclohexane dispersed within an emulsifying medium (1988)
    Springer
    Journal of thermal analysis and calorimetry 34 (1988), S. 1083-1092 
    Details
    ISSN: 1572-8943
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Neue Differential-Scanning-Kalorimetrie- und Röntgendiffraktionsexperimente an Phasenübergangen vpn in einem emulsionsbildendem Medium (Mikroproben von 1 μm3 Volumen) dispergierten Cyclohexans werden dargestellt. Neben den schon bekannten Phasen (zwei stabile Phasenα undγ sowie zwei metastabile phasenβ undδ) wird die Existenz einer dritten metastabilen Phaseε nachgewiesen. Ausserdem werden Interpretationen über die Reihenfolge der Übergänge zwischen diesen verschiedenen Phasen und der flüssigen Phase in Abhängigkeit von Abkühlen und Erwärmen gegeben.
    Abstract: Резюме Представлены данные ДСК измерений и диффракции рентгено вских лучей по фазовому превращени ю циклогексана, диспергированного в среде эмульгатора. На ряду с уже известными фазами ци клогексана (две стаби льные фазы а и у и две метастабильн ые фазыβ иδ), установлено существ ование третьей метас табильной кристаллической фаз ы, обозначаемой е. Приведены объяснени я, касающиеся последо вательности превращения между эт ими различными фазами, а т акже с жидкой фазой пр и нагревании или охлаж дении.
    Notes: Abstract Differential scanning calorimetry and X-ray diffraction experiments on the phase transformations of cyclohexane dispersed within an emulsifying medium (microsamples 1 μm3 in volume) are presented. The existence of a third metastable crystalline phase (denotedε) besides the already-known phases (two stable phases,α andγ, and two metastable phases,β andδ) is demonstrated. Interpretations of the succession of transformations between these different phases and with the liquid phase, either upon cooling or upon heating, are given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01913512
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