ISSN:
1432-072X
Schlagwort(e):
Alicyclic compounds
;
Denitrification
;
Cyclohexanol dehydrogenase
;
Cyclohexanone dehydrogenase
;
2-Cyclohexenone hydratase
;
3-Hydroxycyclohexanone dehydrogenase
;
1,3-Cyclohexanedione hydrolase
;
Phenol
;
Aromatization
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract The enzymes involved in the anaerobic degration of cyclohexanol were searched for in a denitrifying Pseudomonas species which metabolizes this alicyclic compound to CO2 anaerobically. All postulated enzyme activities were demonstrated in vitro with sufficient specific activities. Cyclohexanol dehydrogenase catalyzes the oxidation of the substrate to cyclohexanone. Cyclohexanone dehydrogenase oxidizes cyclohexanone to 2-cyclohexenone. 2-Cyclohexenone hydratase and 3-hydroxycyclohexanone dehydrogenase convert 2-cyclohexenone via 3-hydroxycyclohexanone into 1,3-cyclohexanedione. Finally, the dione is cleaved by 1,3-cyclohexanedione hydrolase into 5-oxocaproic acid. Some kinetic and regulatory properties of these enzymes were studied.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00409663
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