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  • 1
    Electronic Resource
    Electronic Resource
    Pathogenicity and growth of Metarhizium anisopliae stably transformed to benomyl resistance (1990)
    Springer
    Current genetics 17 (1990), S. 129-132 
    Details
    ISSN: 1432-0983
    Keywords: Metarhizium anisopliae ; Benomyl-resistance ; Transformation ; Entomopathogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The insect pathogenic hyphomycete Metarhizium anisopliae was transformed to benomyl resistance using pBENA3, a plasmid containing the benA3 allele from Aspergillus nidulans. The transformation rate was 9 transformants/50 μg DNA/2×106 viable protoplasts. Southern hybridization analyses indicated that the plasmid integrated by nonhomologous recombination at multiple loci. The sites of integration differed among transformants. There was no evidence for autonomous plasmid replication in the transformants. Transformants grew at benomyl concentrations up to 10 times that which inhibits wild type, and they were mitotically stable on either selective or non-selective medium or insect tissue. The transformants were pathogenic to the hornworm, Manduca sexta, producing both appressoria and the cuticle-degrading enzyme, chymoelastase, in the presence of 50 μg/ml of benomyl. These studies demonstrate the potential of using transgenic strains of entomopathogenic fungi along with other components of pest control such as fungicides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312857
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Role of NMDA and non-NMDA receptors in synaptic transmission in rat piriform cortex (1990)
    Springer
    Experimental brain research 82 (1990), S. 451-455 
    Details
    ISSN: 1432-1106
    Keywords: Glutamate receptors ; NMDA receptor ; Olfactory cortex ; DNQX ; AP5 ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The pharmacology of synaptic transmission was studied in slices of rat piriform cortex using the selective non-NMDA glutamate receptor antagonist 6,7-dinitroquinoxaline-2,3-dione (DNQX) and the selective NMDA receptor antagonist D-2-amino-5-phosphonopentanoate (D-AP5). DNQX produced a dose-dependent blockade of synaptic transmission at both lateral olfactory tract and associational system synapses with half-maximal effects at about 2.5 μM. D-AP5 had no significant effects on field potentials recorded in medium containing 2.5 mM Mg++. However in low Mg++ (100–200 μM) medium, D-AP5 did reduce a slow component of postsynaptic responses in both synaptic systems. In Mg++-free medium, 20 μM DNQX did not completely block transmission; the remaining response components were blocked by D-AP5. These results suggest that normal synaptic transmission in the two main inputs to the superficial layers of piriform cortex is mediated by non-NMDA receptors but that NMDA receptors can also participate under conditions where the Mg++ block of the NMDA channel is alleviated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231264
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of an amino acid substitution in the benA, β-tubulin gene of Aspergillus nidulans that confers thiabendazole resistance and benomyl supersensitivity (1990)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 17 (1990), S. 87-94 
    Details
    ISSN: 0886-1544
    Keywords: benzimidazole ; anti-microtubule agents ; carbendazim ; nocodazole ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We are using molecular genetic techniques to identify sites of interaction β-tubulin with benzimidizole anti-microtubule agents. We have developed a marker-rescue technique for cloning mutant alleles of the benA, β-tubulin gene of Aspergillus nidulans and have used the technique to clone two mutant benA alleles, benA16 and benA19. These are the only A. nidulans alleles known to confer resistance to the benzimidazole antimicrotubule agent thiabendazole and supersensitivity to other benzimidazole antimicrotubule agents including benomyl and its active breakdown product, carbendazim. benA16 has been shown, moreover, to reduce thiabendazole binding to β-tubulin. We have sequenced the two mutant alleles and have found that they carry different nucleotide changes that cause the same single amino acid substitution, valine for alanine at amino acid 165. Since thiabendazole and carbendazim differ at only one side chain, the R2 group, we conclude that the region around amino acid 165 is involved in the binding of the R2 group of benzimidazole antimicrotubule agents to β-tubulin.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970170204
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Mechanismus der Entmischung von Chromindiumsulfid-Spinellmischkristallen. Transmissionselektronenmikroskopische Untersuchungen (HRTEM) (1990)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 589 (1990), S. 122-128 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Mechanism of the Decomposition of Chromium Indium Sulfide Spinel Solid Solutions - Transmission Electron Microscope Studies (HRTEM)The mechanism of the probably spinodal decomposition of the spinel type solid solutions MCr2-2xIn2xS4 with M = Fe, Co below 850 and 1000°C, respectively, was studied by high resolution transmission electron microscope technique (HRTEM). The decomposition starts by migration of the metal ions in the tetrahedral 8a sites of the spinel structure to the 16c interstitial sites forming lamellar domains of NaCl structure and NaCl superstructure (Zr3S4 or LiVO2 type), respectively.
    Notes: Der Mechanismus der wahrscheinlich spinodalen Entmischung von Spinellmischkristallen des Typs MCr2-2xIn2xS4 (M = Fe, Co) unterhalb 850 bzw. 1000°C wurde mit Hilfe hochauflösender transmissionselektronenmikroskopischer Messungen (HRTEM) untersucht. Die Entmischung erfolgt primär durch Platzwechsel der tetraedrisch koordinierten Metallionen (8a-Lagen der Spinellstruktur) auf oktaedrische 16c-Zwischengitterpositionen unter Ausbildung von lamellaren Domänen mit NaCl-Struktur bzw. NaCl-Überstruktur (Zr3S4- bzw. LiVO2-Typ).
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19905890114
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    Paper (German National Licenses)
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