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  • 1990-1994  (2)
  • 1990  (2)
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  • 1990-1994  (2)
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  • 1
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have used a bovine papilloma virus (BPV) based mammalian cell expression vector consisting of the complete BPV genome and a human cytomegalovirus transcription unit for the production of soluble CD4. Mouse C-127 cells were transfected with vector DNA together with a selectable G418 resistance plasmid. Surviving clones were selected for high production using a solid phase ELISA based on the immobilization of supernatant-derived CD4 onto nitrocellulose paper and subsequent detection with anti-CD4 antibodies. The expressed protein was shown to bind HIV-gp120 and efficiently block HIV-1 infection in vitro. The possibility to use the above system for rapid production of defined glycoprotein fragments harboring defined functional regions, for the further elucidation of the functional role of CD4 in antigen presentation and cell to cell contact, and for possible intervention during HIV infection is discussed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In einer retrospektiven Studie wurden Häufigkeit und klinische Verläufe der akuten B-Hepatitis mit und ohne Koinfektion durch Hepatitis D Virus (HDV) in der Region Stockholm während zwei verschiedener Zeitphasen, September 1977 bis Oktober 1978 und November 1984 bis Oktober 1986, untersucht. Eine HDV-Koinfektion war bei 31/229 Patienten mit akuter HBV-Infektion (14%) aufgetreten. Zwischen den 70er und 80er Jahren waren keine Unterschiede in der Häufigkeit der Koinfektionen, die bei 12% und 15% lagen, festzustellen. 74% der 31 zusätzlich mit HDV infizierten Patienten waren i. v. Drogenabhängige. Insgesamt hatten 23/66 i. v. Drogenabhängigen (35%), die an einer akuten HBV-Infektion erkrankt waren, eine HDV-Koinfektion. Bei 63% der zusätzlich mit HDV infizierten Patienten war ein zweiphasischer Anstieg von Alanin Aminotransferase und Bilirubin im Serum zu beobachten; dies war nur bei 8% der nur mit HBV Infizierten der Fall (p〈0,001). Ein schwerer klinischer Verlauf der Hepatitis fand sich bei Patienten mit HDV-Koinfektion signifikant häufiger als bei Kranken, die nur durch HBV infiziert waren.
    Notes: Summary The frequency and clinical features of acute hepatitis B virus (HBV) infection with and without a hepatitis D virus (HDV) co-infection was investigated retrospectively in the Stockholm region during two different time periods, September 1977–October 1978 and November 1984–October 1986. Totally, 31/229 (14%) patients with acute HBV infection had a HDV co-infection. No change in the frequency of co-infections, 12% and 15%, respectively, was observed between the 1970s and 1980s. Among the 31 HDV co-infected patients 74% were intravenous drug addicts. Totally 23/66 (35%) intravenous drug addicts with acute HBV infection had HDV co-infection. Clinically a biphasic rise of the serum levels of alanine aminotransferase and bilirubin was noted among 63% of the HDV co-infected patients but only among 8% of the solely HBV infected patients (p〈0.001). A clinically more severe hepatitis was seen significantly more often among the HDV co-infected patients than among the solely HBV infected.
    Type of Medium: Electronic Resource
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