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  • 1990-1994  (3)
  • 1965-1969
  • 1992  (3)
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  • 1990-1994  (3)
  • 1965-1969
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Cryopreservation of in vitro-cultured multiple bud clusters of asparagus (Asparagus officinalis L. cv Hiroshimagreen (2n=30) by the techniques of vitrification (1992)
    Springer
    Plant cell reports 11 (1992), S. 433-437 
    Details
    ISSN: 1432-203X
    Keywords: Cryopreservation ; Vitrification ; Asparagus ; In vitro ; cultured bud clusters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A culture line of asparagus forming green bulbous structures consisting of numerous multiple bud clusters designated “bud clusters” was induced from a meristem culture of asparagus (Asparagus officinalis L.cv. Hiroshimagreen, 2n=30). Small cubic segments (2 mm3) cut from bud clusters were cryopreserved using three different cryogenic protocols. Only vitrification produced very high levels of shoot formation after cooling to −196°C. Segments were treated with a vitrification solution (PVS2) at 25°C for 45 min or at 0°C for 120 min prior to a direct plunge into liquid nitrogen. After rapid warming, the segments were expelled into Murashige and Skoog medium containing 1.2 M sucrose for 10 min and then plated on agar shoot outgrowth medium. The average rate of shoot formation of vitrified segments producing normal shoots was near 90% without any preculture and/or cold-acclimation treatment. Revived segments resumed growth within 3 days and developed about three shoots per segment. In vitro-cultured bud clusters appear promising as material for cryopreserving asparagus germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232685
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cryopreservation of in vitro-grown shoot tips of apple and pear by vitrification (1992)
    Springer
    Plant cell, tissue and organ culture 28 (1992), S. 261-266 
    Details
    ISSN: 1573-5044
    Keywords: apple ; cryopreservation ; fruit trees ; Malus ; pear ; Pyrus ; shoot tips ; vitrification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vitro-grown shoot tips of apples (Malus domestica Borkh. cv. Fuji) were successfully cryopreserved by vitrification. Three-week-old in vitro apple plantlets were cold-hardened at 5°C for 3 weeks. Excised shoot tips from hardened plantlets were precultured on a solidified Murashige & Skoog agar medium (MS) supplemented with 0.7 M sucrose for 1 day at 5°C. Following preculture shoot tips were transferred to a 2 ml plastic cryotube and a highly concentrated cryoprotective solution (designated PVS2) was then added at 25°C. The PVS2 contains (W/V) 30% glycerol, 15% ethylene glycol and 15% dimethylsulfoxide in medium containing 0.4 M sucrose. After dehydration at 25°C for 80 min, the shoot tips were directly plunged into liquid nitrogen. After rapid warming, the shoot tips were expelled into 2 ml of MS medium containing 1.2 M sucrose and then plated on agar MS medium. Direct shoot elongation was observed in approximately 3 weeks. The average rate of shoot formation was about 80%. This vitrification method was successfully applied to five apple species or cultivars and eight pear cultivars. This method appears to be a promising technique for cryopreserving shoot tips from in vitro-grown plantlets of fruit trees.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036122
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Pulsed chemical vapour infiltration of SiC to three-dimensional carbon fibre preforms (1992)
    Springer
    Journal of materials science 27 (1992), S. 6022-6028 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Three-dimensional carbon fibre preforms were infiltrated with silicon carbide from a gas system of CH3SiCl3-H2 using a process of pressure pulsed chemical vapour infiltration. To infiltrate to a deep level, the temperature had to be lowered to 870–900°C, and the hold time per pulse below 1.0 s. Three-dimensional carbon fibre preforms partly filled with SiC fine powder were compared with those without filler. The weight of the preforms increased linearly with increasing number of pulses up to 105 when no filler was present. However, the weight increase slowed down above 8×104 pulses when the filler was used. Preforms with and without SiC filler showed three-point flexural strengths of 160 and 80 MPa after CVI of 105 pulses, respectively. In order to improve the strength, a denser filling of SiC powder is necessary.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01133744
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    Paper (German National Licenses)
    Fulltext
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