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  • 1
    Electronic Resource
    Electronic Resource
    Differential depolarization-activated calcium responses in fetal and neonatal rat osteoblast-like cells (1994)
    Springer
    Calcified tissue international 54 (1994), S. 278-283 
    Details
    ISSN: 1432-0827
    Keywords: Calcium channels ; Development ; Osteoblastic cells ; Fura-2 ; Patch-clamp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract The present study evaluates differential occurrence of voltage-dependent calcium channels (VDCC) in the membranes of fetal (FROB) and neonatal (NROB) calvarian rat osteoblastic cells in primary culture. The intracellular calcium concentration ([Ca2+]i) was monitored upon depolarization of the cell membrane with the use of high K+ containing extracellular solutions. [Ca2+]i was measured in populations of cells as well as in individual cells using Fura-2, whereas the membrane potential (Em) was recorded in parallel experiments using patch-clamp techniques. Increasing the extracellular K+ concentration resulted in an instantaneous depolarization of Em of both FROB and NROB. This depolarization of Em did not significantly affect [Ca2+]i of populations of FROB and neonatal osteoblast precursors (NpROB). In contrast to FROB and NpROB, NROB populations responded to depolarization with significant transient [Ca2+]i increases that could be blocked by the calcium antagonist verapamil and were absent if extracellular Na+ was replaced for choline instead of K+. In individual cell measurements, response frequencies as well as the magnitude of [Ca2+]i responses upon depolarization of NROB were much higher than those of FROB, suggesting that more NROB than FROB possess VDCC. This phenomenon might point to a development-related expression of VDCC in the membranes of osteoblast-like cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00295951
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    1:2 complexes of (thio)morpholinium derivatives and the electron acceptor 7,7,8,8-tetracyano-p-quinodimethane. VII. Triclinic form of N,N-dimethylmorpholinium di-7,7,8,8-tetracyano-p-quinodimethanide, DMM(TCNQ)2 (II), at 294 K (1994)
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 50 (1994), S. 1139-1141 
    Details
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108270193011199
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Risks and benefits of antireflux operations in neurologically impaired children (1994)
    Springer
    European journal of pediatrics 153 (1994), S. 248-251 
    Details
    ISSN: 1432-1076
    Keywords: Key words: Gastro-oesophageal reflux – Neurologically impaired children – Anterior gastropexy – Gastrostomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Gastro-oesophageal reflux (GER) in neurologically impaired children often causes feeding problems and complications of oesophagitis and is frequently resistant to medical treatment. Fifty neurologically impaired children underwent anterior gastropexy as anti-reflux operation, combined with gastrostomy in 23, between 1976 and 1992. There was no operative mortality. There were 25 early complications in 14 patients and 9 late complications in 9 patients. Twelve patients needed 17 re-operations for delayed gastric emptying [4], intestinal obstruction [3], para-oesophageal hernia [3], oesophageal stenosis [4], and recurrent GER, revision of gastrostomy, subphrenic abcess (one each). Nine patients died during the follow-up period. Death in two children was related to the operation (incarcerated para-oesophageal hernia and blow-out of the stomach). Out of 41 survivors, the operation was judged successful in 35. It is concluded that antireflux operations in neurologically impaired children carry a high risk of complications. Preoperative identification of risk factors is not possible. The improvements in the quality of life achieved in the majority of patients outweigh the risks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01954512
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Risks and benefits of antireflux operations in neurologically impaired children (1994)
    Springer
    European journal of pediatrics 153 (1994), S. 248-251 
    Details
    ISSN: 1432-1076
    Keywords: Gastro-oesophageal reflux ; Neurologically impaired children ; Anterior gastropexy Gastrostomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Gastro-oesophageal reflux (GER) in neurologically impaired children often causes feeding problems and complications of oesophagitis and is frequently resistant to medical treatment. Fifty neurologically impaired children underwent anterior gastropexy as anti-reflux operation, combined with gastrostomy in 23, between 1976 and 1992. There was no operative mortality. There were 25 early complications in 14 patients and 9 late complications in 9 patients. Twelve patients needed 17 re-operations for delayed gastric emptying [4], intestinal obstruction [3], para-oesophageal hernia [3], oesophageal stenosis [4], and recurrent GER, revision of gastrostomy, subphrenic abcess (one each). Nine patients died during the follow up period. Death in two children was related to the operation (incarcerated para-oesophageal hernia and blowout of the stomach). Out of 41 survivors, the operation was judged successful in 35. It is concluded that antireflux operations in neurologically impaired children carry a high risk of complications. Preoperative identification of risk factors is not possible. The improvements in the quality of life achieved in the majority of patients outweigh the risks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01954512
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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