ISSN:
1573-5176
Keywords:
carrageenophyte
;
cytophotometry
;
Gigartinales
;
Hypnea
;
nuclear genome
;
reassociation kinetics
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract DNA reassociation kinetics were used to determine nuclear genome organization and complexity in the carrageenophyteHypnea musciformis (Gigartinales, Rhodophyta). Results indicate the presence of three second order components corresponding to fast (2%), intermediate (33%) and slow (65%) fractions. Microspectrophotometry with the DNA-localizing fluorochrome DAPI confirmed ploidy level differences in the gametophytic and tetrasporophytic phases. Elevated (endopolyploid) nuclear DNA levels were observed in both gametophytic and cystocarpic tissue. Comparison of mean nuclear DNA (If) values to chicken erthrocytes (RBC) resulted in an estimate of 0.22 pg/2 C genome forHypnea musciformis. Karyological studies using aceto-orcein revealed a chromosome complement of five bivalents during diakinesis of tetraspore mother cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02185897
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