ISSN:
1573-6849
Keywords:
base-specific dyes
;
DNA content
;
Pleurodeles
;
sex chromosomes
;
video-imaging fluorescence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The mitotic Z and W sex chromosomes inPleurodeles seem to be identical. Earlier morphological and molecular analyses of lampbrush paired chromosomes in the female meiosis showed clearly that 20% of the chromosomal length located in the middle part of the sex bivalent (bivalent IV) is heteromorphic. We investigated here the base content and composition of the DNA axes in the heteromorphic region by quantitative fluorescence imaging using various base-specific (DAPI, Hoechst 33342 and chromo-mycin A3) or base-nonspecific (ethidium bromide) fluorescent DNA probes. Our results show a significantly higher percentage of AT bases in Z than in W differential sectors. In addition the entire base content of Z appears slightly higher than that of W.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01553324
Permalink