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  • 1995-1999  (24)
  • 1930-1934
  • 1999  (1)
  • 1995  (23)
  • Polymer and Materials Science  (20)
  • gene expression  (3)
  • hepatectomy
Material
Years
  • 1995-1999  (24)
  • 1930-1934
Year
  • 1
    ISSN: 1436-2813
    Keywords: hepatocellular carcinoma ; lupoid hepatitis ; hepatectomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A 66-year-old woman was diagnosed as having lupoid hepatitis due to the presence of hypergammaglobulinemia, lupus erythematosus cells, and positivity for antinuclear, anti-DNA, and anti-smooth muscle antibodies. None of the serum hepatitis B markers were positive. Symptomatic relief was obtained by prednisolone administration. Five years after the diagnosis of lupoid hepatitis, hepatocellular carcinoma (HCC) was detected by ultrasonography and computed tomography, after which hepatectomy was performed. Although transcatheter arterial embolization was done on two occasions and repeat hepatectomy was performed twice for recurrent HCC, her liver function remained good with the prednisolone treatment. Antibody for hepatitis C virus has been negative since our first check in 1992. As of this writing, the patient has been alive and well 6 years and 2 months after the first hepatectomy. There have been no previous reports of 6-year survival after hepatectomy for HCC associated with lupoid hepatitis.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5028
    Keywords: glycine betaine ; betaine aldehyde dehydrogenase ; osmotic stress ; gene expression ; plant hormone ; abscisic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When subjected to salt stress or drought, some vascular plants such as barley respond with an increased accumulation of the osmoprotectant glycine betaine (betaine), being the last step of betaine synthesis catalyzed by betaine aldehyde dehydrogenase (BADH). We report here cloning and characterization of BADH cDNA from barley, a monocot, and the expression pattern of a BADH transcript. An open reading frame of 1515 bp encoded a protein which showed high homology to BADH enzymes present in other plants (spinach and sugar-beet) and in Escherichia coli. Transgenic tobacco plants harboring the clone expressed high levels of both BADH protein and its enzymatic activity. Northern blot analyses indicated that BADH mRNA levels increased almost 8-fold and 2-fold, respectively, in leaves and roots of barley plants grown in high-salt conditions, and that these levels decreased upon release of the stress, whereas they did not decrease under continuous salt stress. BADH transcripts also accumulate in response to water stress or drought, indicating a common response of the plant to osmotic changes that affect its water status. The addition of abscisic acid (ABA) to plants during growth also increased the levels of BADH transcripts dramatically, although the response was delayed when compared to that found for salt-stressed plants. Removal of plant roots before transferring the plants to high-salt conditions reduced only slightly the accumulation of BADH transcripts in the leaves.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; chloroplast ; gene expression ; ω-3 fatty acid desaturase ; promoter ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Arabidopsis FAD7 gene encodes a chloroplast ω-3 fatty acid desaturase that catalyzes the desaturation of lipid-linked dienoic fatty acids (18:2 and 16:2). An 825 bp FAD7 promoter fragment upstream from the transcriptional start point contained several short sequences which were homologous to the cis-elements (box II, G-box, etc.) conserved in many light-responsive genes. We introduced the FAD7 promoter fused to the β-glucuronidase (GUS) or the luciferase (LUC) reporter gene into tobacco plants. The −825 promoter sequence conferred tissue-specific and light-responsive expression to both these reporter genes in transgenic tobacco, indicating that these expressions of the FAD7 gene were regulated mainly at the transcriptional level. Histochemical GUS staining showed that the activity of the FAD7 promoter is restricted to the tissues with chloroplast-containing cells although the staining was noticeably absent in the chloroplast-containing cells associated with vascular systems. The 5′ deletion experiments of the promoter revealed that the −362/ −166 region, containing two putative box II sequences, was responsible for the tissue-specific and light-responsive expression of the FAD7 gene.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-7241
    Keywords: ventricular remodeling ; myocardial infarction ; diltiazem ; Doppler echocardiography ; gene expression ; cardiac function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Summary. The purpose of this study was to examine the effect of diltiazem on cardiac dysfunction and the change in cardiac gene expression after myocardial infarction in rats. On the first day after myocardial infarction, rats were randomly assigned to a diltiazem treatment (Dil, n = 7) or an untreated group (MI, n = 8). We then performed Doppler-echocardiographic examinations on the rats and measured their hemodynamics at 4 weeks after myocardial infarction. Following these measurements, their cardiac mRNA was analyzed. Diltiazem decreased the mean aortic pressure and heart rate. Left ventricular end-diastolic pressure (LVEDP) and central venous pressure (CVP) increased to 18 ± 2 mmHg and 5 ± 1 mmHg (P 〈 0.01). Diltiazem reduced LVEDP to 14 ± 1 mmHg (P 〈 0.05), but it did not change CVP. The weight of the right ventricle in MI was significantly larger than in the control rats (control, n = 7, 0.46 ± 0.02 g/kg vs. MI, 0.81 ± 0.06 g/kg; P 〈 0.01). The left ventricular end-diastolic dimension (LVDd) in MI increased to 8.8 ± 0.3 mm (P 〈 0.01, control, 6.1 ± 0.3 mm). Diltiazem prevented an increase in the weight of the right ventricle (0.69 ± 0.03 g/kg, P 〈 0.05) and LVDd (7.7 ±6 0.2 mm, P 〈 0.05 to MI). The rats within MI showed systolic dysfunction, defined by a decreased ejection fraction (control, 67 ± 2% vs. MI, 36 ± 3%, P 〈 0.01), and diastolic dysfunction, defined by the E-wave deceleration rate (control, 13.4 ± 1.6 m/s2 vs. MI, 30.4 ± 3.4 m/s2 P 〈 0.01). Diltiazem significantly prevented systolic and diastolic dysfunction. The increases in β-MHC, ANP, and collagen type I and III mRNAs in the noninfarcted left ventricle and right ventricle were significantly suppressed by treatment with diltiazem. α-Skeletal actin increased in MI, and α-skeletal actin was more increased with Dil. In conclusion, diltiazem prevents cardiac dysfunction and morphological change due to left ventricular remodeling after experimental myocardial infarction.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The Cd(II) complex of a peptide, Z-Cys-Ala-Pro-His-OMe was prepared and characterized by absorption, CD, 1H-, 13C-, and 113Cd-nmr, and nuclear Overhauser effect spectroscopy (NOESY) spectra to show the coordination of cysteine thiolate and histidine imizazole to Cd(II) ion. The NOESY spectra in dimethyl formamide showed that the cysteine residue was in proximity to the histidine residue. These results reveal the dictation of Z-Cys-Ala-Pro-His-OMe to Cd(II) ion in solution. Temperature-dependent dissociation equilibrium of histidine imidazole in solution was observed in this complex. Structural features of the chelating peptide are discussed. © 1995 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 33 (1995), S. 2657-2665 
    ISSN: 0887-624X
    Keywords: amphiphilic rubber ; butyl rubber ; saccharide ; microphase-separated structure ; thermoplastic elastomer ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A new type of amphiphilic elastomer was synthesized, which consisted of butyl rubber and a D-maltose derivative as a backbone and side groups, respectively. The synthesis was accomplished by the sequence of the three following reactions: (1) Hydroxyl groups of D-maltonolactone were protected by a trimethylsilyl group. (2) The resulting maltonolactone derivative was subjected to a reaction with chemically modified butyl rubber having pendant amino groups. (3) The protecting trimethylsilyl groups on the maltose residues were removed by treating with tetra-n-butylammonium fluoride. This new amphiphilic elastomer behaved as a thermoplastic elastomer and showed good mechanical properties. The saccharide seg-ments aggregated in the hydrophobic butyl rubber matrix to form a microphase-separated structure, as confirmed by differential scanning calorimetry, transmission electron mi-croscopy, small-angle x-ray scattering, and dynamic mechanical measurements. The ag-gregated saccharide domains are estimated to function both as crosslinking sites and rein-forcing fillers in the rubber matrix. © 1995 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 33 (1995), S. 2157-2163 
    ISSN: 0887-624X
    Keywords: reactive polyester ; pendant carboxyl group ; chemoselective polycondensation ; polymer catalyst ; polymer reaction ; bifunctional catalytic mechanism ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Aromatic polyester, copolyester, and poly(ester-amide-thioester) having pendant carboxyl groups are directly synthesized by the organic phase/water phase interfacial polyconden-sation using low-molecular and polymeric phase transfer catalysts. Spectral analysis of the resulting polymers indicates that the nucleophilicity of salts of phenols to diacid chloride is far higher than that of salts of carboxylic acids and chemoselective esterification occurs in a 100% yield. Even if the polymeric catalyst having amino acid moiety as a nucleophilic group is used in the polycondensation, the polymers do not contain anhydride groups. The polyester can be almost quantitatively converted to polymers with different reactive groups by reacting the pendant carboxyl groups with alkyl halides in a DMAc-H2O mixture con-taining K2CO3. A bifunctional catalytic mechanism is proposed for the chemical modification of the polyesters. © 1995 John Wiley & Sons, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 33 (1995), S. 2027-2031 
    ISSN: 0887-624X
    Keywords: reactive polyester ; organic/organic interface ; interfacial polycondensation ; reaction mechanism ; solvent participation ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Copolycondensation of isophthaloyl chloride, bisphenol A, and aliphatic diols with additional reactive groups were performed in the presence of triethylamine by a trimethyl phosphate/cyclohexane organic/organic interfacial method. The composition of idol and bisphenol A residues in the resulting copolyesters is very close to that in the feed from the initial stage of reaction. The resulting copolyesters with reactive groups can be used for the preparation of various functional polymers. The mechanism of an organic/organic interfacial polycondensation was also discussed. © 1995 John Wiley & Sons, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Electroanalysis 7 (1995), S. 884-888 
    ISSN: 1040-0397
    Keywords: Potentiometric sensors ; Nonaqueous media ; ISFET ; pH ; Proton solvation ; Lithium ion solvation ; Liquid junction potential ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Four recent studies in the authors' laboratory on the use of electrochemical sensors in nonaqueous solutions are reported. (1) Si3N4- and Ta2O5-type ISFETs proved to be useful in nonaqueous solutions as sensors for pH and for proton solvation, because of their more rapid response than a conventional glass electrode. They were used to study the acid-base equilibria in γ-butyrolactone and to determine the proton transfer activity coefficients between different solvents. (2) A univalent cation-sensitive glass electrode was used to study the solvation of lithium ions in mixed solvents applicable to lithium batteries. (3) A membrane of polyacrylamide coupled to a cobalt phthalocyanine complex and coated onto a platinum electrode functioned as a sensor for fluoride and cyanide ions in aprotic solvents. It was used to determine the solubility products of alkali and alkaline earth metal fluorides and cyanides in acetonitrile and propylene carbonate. (4) A new method is proposed for the estimation of the liquid junction potential between different solvents, and a comparative study has been made between the new method and some conventional methods.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 33 (1995), S. 159-165 
    ISSN: 0887-6266
    Keywords: dyeing theory ; multimodal sorption ; nonionic dyes ; bimodal langmuir sorption ; nernst-type partitioning ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Sorption isotherms of nonionic dyes with two amino groups (one anthraquinone dye and two azo dyes) on various polymers from water were measured at 40-90°C (Nylon-6 and cellulose film) and at 95°C (polyester microfiber). The isotherms were curved, convex to upward, in the range of low dye concentration Cs in water and almost linear in the range of medium to saturated Cs. The isotherms measured at low temperature (40°C for cellulose, 40-60°C for Nylon-6, and at 95°C for polyester) were satisfactorily described by considering three concurrent modes of sorption. They are Nernst type partitioning and bimodal Langmuir sorption (sorption by the higher affinity sites with a small saturation value and that by the lower affinity sites with a large saturation value). However, for the sorption of the anthraquinone dye and one azo dye by Nylon-6 film at high temperature (80-90°C), the amount of dye sorbed by the high affinity site decreased to negligibly small. Accordingly, the isotherms were expressed well by simple dual-sorption model. © 1995 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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