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  • 1
    ISSN: 1432-2048
    Keywords: Aldoxime biosynthesis ; Auxin biosynthesis ; Brassica ; Glucosinolate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The first steps in the biosynthesis of glucosinolates and indole-3-acetic acid (IAA) in oilseed rape (Brassica napus L.) and Chinese cabbage (Brassica campestris ssp. pekinensis) involve the formation of aldoximes. In rape the formation of aldoximes from chain-extended amino acids, for aromatic and aliphatic glucosinolate biosynthesis, is catalysed by microsomal flavin-containing monooxygenases. The formation of indole-3-aldoxime from l-tryptophan, the potential precursor of both indole-3-acetic acid and indolyl-glucosinolates, is catalysed by several microsomal peroxidases. The biosynthesis of glucosinolates and indole-3-acetic acid was shown to be under developmental control in oilseed rape and Chinese cabbage. No monooxygenase activities were detected in cotyledons or old leaves of either species. The highest monooxygenase activities were found in young expanding leaves; as the leaves reached full expansion and matured the activities decreased rapidly. The indole-aldoxime-forming activity was found in all of the tissues analysed, but there was also a clear decrease in foliar activity with maturity in leaves of rape and Chinese cabbage. Partial characterisation of the Chinese cabbage monooxygenases showed that they have essentially identical properties to the previously characterised rape enzymes; they are not cytochrome P450-type enzymes, but resemble flavin-containing monooxygenases. No monooxygenase inhibitors were detected in microsomes prepared from either cotyledons or old leaves.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5117
    Keywords: macrophytes ; streams ; PCA ; HAC ; bio-indicator ; water chemistry ; geology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aquatic vegetation of twenty one streams, located at the head of watersheds and representative of the geological diversity of the Lorraine, was analysed with regard to its environmental d escriptors (geology, morphometry and water chemistry). A Principal Components Analysis was carried out on the data (230 stations and 134 variables). using 40 active chemical and morphometrical variables. This procedure was followed by a Hierarchical Ascending Classification for grouping the variables into classes. The analysis of the mesological and chemical structures of the streams indicated the dominance of geochemical parameters compared to pollution parameters. The indicator capacity of several species was revealed, e.g. Scapania undulata for weakly mineralized, oligotrophic waters; Potamogeton pectinatus for eutrophicated waters and Ranunculus fluitans for high nitrate levels. Five species groups characterizing the geology and mineralization as well as the perturbations in the quality of the water or the environment were identified (e.g. pollution, eutrophication, reposition and reshaping).
    Type of Medium: Electronic Resource
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