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  • 1995-1999  (5)
  • 1995  (5)
  • Cultured DRG neurones  (2)
  • Neurospora  (2)
  • glycosylation
  • 1
    Electronic Resource
    Electronic Resource
    An electrophysiological investigation of the effects of cisplatin and the protective actions of dexamethasone on cultured dorsal root ganglion neurones from neonatal rats (1995)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 352 (1995), S. 247-255 
    Details
    ISSN: 1432-1912
    Keywords: Key words: Cisplatin ; Dexamethasone ; Calcium-dependent currents ; Voltage-activated potassium currents ; Cultured DRG neurones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  In this study we have investigated the acute and chronic effects of cisplatin on whole cell currents in cultured dorsal root ganglion neurones. Consistent with effects on action potentials measured under current clamp, acute (5 min) application of cisplatin (5 μM) attenuated voltage-activated potassium, and mixed cation currents by approximately 50% in both cases. Chronic treatment (5–7 days) of cultured neurones with 5 μM cisplatin also resulted in greatly reduced voltage-activated potassium currents (by 50%) and calcium currents (by 60%) compared to events recorded from neurones not treated with cisplatin. In contrast, the amplitude of inward cation current activated by hyperpolarization was doubled by 5–12 days treatment with cisplatin. Studies on action potential after-depolarizations and calcium-activated chloride currents suggest that cisplatin disturbs calcium homeostatic mechanisms. These observations may account for anode break spike excitation and the low efficiency with which cells buffer intracellular calcium following cisplatin treatment. Dexamethasone has been found to enhance the anti-emetic effects of 5-HT3 receptor antagonists in patients treated with cisplatin. For this reason the actions of dexamethasone were studied in combination with cisplatin treatment. Although acute application of dexamethasone (1–10 μM) produced transient depolarizations and bursts of action potentials, after 5 minutes application it had no effect on membrane potential, input resistance, or the properties of action potentials evoked by depolarizing current commands. Compared to cells exposed to cisplatin alone, combined treatment of cisplatin and dexamethasone significantly improved survival of dorsal root ganglion neurones in culture by 20% . Dexamethasone also showed signs of protecting neurones from cisplatin by improving membrane potentials and action potential thresholds. In conclusion, cisplatin reduces the viability of dorsal root ganglion neurones in culture and alters their electrophysiological properties. Evidence suggests that dexamethasone has some protective properties against the neurotoxic actions of cisplatin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00168554
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    An electrophysiological investigation of the effects of cisplatin and the protective actions of dexamethasone on cultured dorsal root ganglion neurones from neonatal rats (1995)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 352 (1995), S. 247-255 
    Details
    ISSN: 1432-1912
    Keywords: Cisplatin ; Dexamethasone calcium ; dependent currents ; Voltage-activated potassium currents ; Cultured DRG neurones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract It this study we have investigated the acute and chronic effects of cisplatin on whole cell currents in cultured dorsal root ganglion neurones. Consistent with effects on action potentials measured under current clamp, acute (5 min) application of cisplatin (5 μM) attenuated voltage-activated potassium, and mixed cation currents by approximately 50% in both cases. Chronic treatment (5–7 days) of cultured neurones with 5 μM cisplatin also resulted in greatly reduced voltage-activated potassium currents (by 50%) and calcium currents (by 60%) compared to events recorded from neurones not treated with cisplatin. In contrast, the amplitude of inward cation current activated by hyperpolarization was doubled by 5–12 days treatment with cisplatin. Studies on action potential after-depolarizations and calcium-activated chloride currents suggest that cisplatin disturbs calcium homeostatic mechanisms. These observations may account for anode break spike excitation and the low efficiency with which cells buffer intracellular calcium following cisplatin treatment. Dexamethasone has been found to enhance the anti-emetic effects of 5-HT3 receptor antagonists in patients treated with cisplatin. For this reason the actions of dexamethasone were studied in combination with cisplatin treatment. Although acute application of dexamethasone (1–10 μM) produced transient depolarizations and bursts of action potentials, after 5 minutes application it had no effect on membrane potential, input resistance, or the properties of action potentials evoked by depolarizing current commands. Compared to cells exposed to cisplatin alone, combined treatment of cisplatin and dexamethasone significantly improved survival of dorsal root ganglion neurones in culture by 20%. Dexamethasone also showed signs of protecting neurones from cisplatin by improving membrane potentials and action potential thresholds. In conclusion, cisplatin reduces the viability of dorsal root ganglion neurones in culture and alters their electrophysiological properties. Evidence suggests that dexamethasone has some protective properties against the neurotoxic actions of cisplatin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00168554
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The dynamics of mitochondrial plasmids in a Hawaiian population of Neurospora intermedia (1995)
    Springer
    Current genetics 29 (1995), S. 44-49 
    Details
    ISSN: 1432-0983
    Keywords: Mitochondrial plasmids ; Senescence ; Neurospora ; Meiotic transmission
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We analysed the distribution of mitochondrial plasmids among 82 Neurospora intermedia isolates from Hawaii; 74% of the isolates carried the neutral circular plasmid Han-2, whereas 38% contained the linear senescence-causing plasmid kalDNA. The distributions of the two plasmids are independent. There is no significant difference between the Kauaian population of 1972 and that of 1976. To further examine the reasons for this frequency distribution we studied the transmission of both Hawaiian plasmids through the maternal parent in a large series of crosses using non-Kalilo isolates as conidial parents. Plasmids can be lost during the sexual cycle. The Han-2 plasmid is transmitted more efficiently than kalDNA. No clear cases of autonomous or non-autonomous plasmid suppression were observed, so loss can be considered accidental. One Kalilo strain proved to be ineffectual as a maternal parent, and this reduced its ability to transmit kalDNA to the next generation. The dynamic balance of plasmids in natural populations over time is probably a result of the interplay of many forces, including those described in this work and those from several other studies on Neurospora plasmids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00313192
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Recombination between heterologous linear and circular mitochondrial plasmids in the fungus Neurospora (1995)
    Springer
    Molecular genetics and genomics 249 (1995), S. 25-36 
    Details
    ISSN: 1617-4623
    Keywords: Neurospora ; Plasmids ; Mitochondria ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A strain of Neurospora intermedia from China contains five prominent extragenomic mitochondrial plasmids: three linear elements called zhisi plasmids, and two circular plasmids, Harbin-1 and -2. In one subculture, levels of four plasmids (all three zhisis and Harbin-1) fell to undetectable values and two novel linear plasmids appeared, Harbin-L and -L2, as well as a new small circular plasmid, Harbin-0.9. Cross-hybridization of restriction fragments and DNA sequencing showed that the Harbin-L plasmid was composed of parts of the circular Harbin-1 plasmid and of one of the linear zhisi plasmids. A model is presented in which the Harbin-1 and zhisi plasmids are present within the same mitochondrion, and crossovers at two separate 7 by sites of sequence identity effectively insert part of the circular Harbin-1 DNA into a zhisi linear plasmid, simultaneously deleting part of the zhisi element. The small plasmid Harbin-0.9 is a fragment of the Har-1 plasmid, and seems to be another product of the recombination process that created Har-L. Recombination of this type could have contributed to the wide array of mitochondrial plasmids found in natural populations of Neurospora.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290232
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    N-glycans of recombinant human interferon-γ change during batch culture of chinese hamster ovary cells (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 48 (1995), S. 639-648 
    Details
    ISSN: 0006-3592
    Keywords: interferon ; glycosylation ; CHO cells ; microheterogeneity ; mass spectrometry ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A recombinant Chinese hamster ovary (CHO) cell line making human interfron-γ (IFN-γ) was grown in 12-L stirred tank fermentors in three batch fermentations under conditions of constant temperature, pH, and dissolved oxygen tension. In addition to cell growth, metabolite, and productivity data, a detailed analysis of the carbohydrate structures attached to each glycosylation site of IFN-γ was achieved using matrix-assisted laser desorption mass spectrometry (MALDI-MS) in combination with exoglycosidase array sequencing. Complex biantennary oligosaccharides (particularly Gal2GlcNAc4Man3 which was core ℵl-6 fucosylated at Asn25 but not at Asng97) were most prevalent at both glycosylation sites. However, considerable microheterogeneity arising from the presence of triantennary and truncated glycan structures was also observed. The proportion of the dominant core glycan structure (Gal2GlcNAc4Man3 ± Fuc1) decreased by 15-26% during batch culture, with increases in the proportion of oligomannose and truncated glycans over the same time period. Prolonged culture resulting from an extended lag phase led to further accumulation of oligomannose and truncated structures, reaching up to 52% of total glycans attached to Asng97 by 240 h of culture. The implications of these glycosylation changes for optimizing the time for harvesting cell cultures, and for the clearance of recombinant therapeutic products in vivo are discussed. © 1995 John Wiley & Sons, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260480612
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    Paper (German National Licenses)
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