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Digitale Medien

Regulation of cell-wall morphology and growth encoded by plasmids in Shigella dysenteriae type 1 (1995)

Biswas, D. ; Ghosh, A. N. ; Kumar, R.

Springer

World journal of microbiology and biotechnology 11 (1995), S. 280-283

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Details

ISSN: 1573-0972

Schlagwort(e): Curing ; electron microscopy ; outer membrane ; plasmids ; SDS-PAGE ; Shigella dysenteriae

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract Shigella dysenteriae type 1, isolated locally, was found to contain six plasmids and these could be eliminated using SDS. A 70-kb plasmid was necessary to maintain the normal cell-wall morphology. Synthesis of nine major membrane proteins (90 to 40 kDa) was severely impaired in all-plasmid cured strains. Electron microscopy revealed a prominent separation between the outer and inner membranes of the cured strains, indicating that plasmid loss led to defects in the cell envelope. The growth rates of the strains having only the 70-kb plasmid and the plasmidless strain were 3- to 30-fold less than in the wild type strain.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00367098

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