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  • 1995-1999  (4)
  • 1985-1989
  • 1996  (4)
  • 1
    Electronic Resource
    Electronic Resource
    A mutant trypsin-like enzyme from Streptomyces fradiae, created by site-directed mutagenesis, improves affinity chromatography for protein trypsin inhibitors (1996)
    Springer
    Applied microbiology and biotechnology 46 (1996), S. 15-21 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The Ser-170 residue of a trypsin-like enzyme from Streptomyces fradiae (SFT), which is considered to be the active-site serine, was replaced with alanine by site-directed mutagenesis to improve the affinity chromatography step for a Kazal-type trypsin inhibitor, pancreatic secretory trypsin inhibitor (PSTI). The resulting mutant SFT, designated as [S170A]SFT, was expressed in Streptomyces lividans and purified to homogeneity. [S170A]SFT was catalytically inactive, but still had the ability to bind tightly to PSTI and to soybean trypsin inhibitor with dissociation constants of 3.1×10-7 M and 1.9×10-8 M respectively. We further demonstrated that recombinant human PSTI secreted into Saccharomyces cerevisiae culture broth could be purified to homogeneity with a one-step [S170A]SFT-affinity column. The purified PSTI contained no molecules intramolecularly cleaved by active trypsin, which are found when trypsin-affinity chromatography is used for the purification. This eliminated the need for further separation of intact PSTI from intramolecularly cleaved PSTI by high-performance liquid chromatography, thus simplifying and improving its purification process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050777
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Optimal design of controlled structures (1996)
    Springer
    Structural and multidisciplinary optimization 11 (1996), S. 19-28 
    Details
    ISSN: 1615-1488
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A formulation that finds the optimal design of a controlled structure is proposed. To achieve this goal, a composite objective composed of structural and control objectives is introduced to be optimized, and the effect of the control weighting is examined. A feedback control law is defined before the structural optimization and then the composite objective will only become a function of structural design variables. As a result, optimal structural design and control forces in steady state are obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279651
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Integrated optimal structural and vibration control design (1996)
    Springer
    Structural and multidisciplinary optimization 12 (1996), S. 209-216 
    Details
    ISSN: 1615-1488
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract An integrated design procedure which is composed of structural design, control design, and actuator locations design is proposed in this paper. First, a composite objective function, formed by a structural and a control objective, is optimized in steady state through the homogenization design method. Then an independent modal space control algorithm (IMSC) is performed on this optimal structure to reduce the dynamic response. Finally, to minimize the control force while still obtaining the same modal response for the controlled modes, the optimal choice for actuator locations is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01197358
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    APPLICATION OF TOPOLOGICAL OPTIMIZATION TECHNIQUES TO STRUCTURAL CRASHWORTHINESS (1996)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 39 (1996), S. 1383-1403 
    Details
    ISSN: 0029-5981
    Keywords: crashworthiness ; homogenization ; topology design ; automotive structure optimization ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The topological optimization of components to maximize crash energy absorption for a given volume is considered. The crash analysis is performed using a DYNA3D finite element analysis. The original solid elements are replaced by ones with holes, the hole size being characterized by a so-called density (measure of the reduced volume). A homogenization method is used to find elastic moduli as a function of this density. Simpler approximations were developed to find plastic moduli and yield stress as functions of density.Optimality criteria were derived from an optimization statement using densities as the design variables. A resizing algorithm was constructed so that the optimality criteria are approximately satisfied. A novel feature is the introduction of an objective function based on strain energies weighted at specified times. Each different choice of weighting factors leads to a different structure, allowing a range of design possibilities to be explored.The method was applied to an automotive body rear rail. The original design and a new design of equal volume with holes were compared for energy absorption.
    Additional Material: 25 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
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