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  • 1995-1999  (2)
  • 1996  (2)
  • Rice  (2)
  • Amino acid sequence
  • pharmacokinetics
  • 1
    Electronic Resource
    Electronic Resource
    Detection of segregation distortions in an indica-japonica rice cross using a high-resolution molecular map (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 145-150 
    Details
    ISSN: 1432-2242
    Keywords: Gametophyte gene ; Genetic map ; Hybrid sterility gene ; Rice ; Segregation distortion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have constructed a high-resolution rice genetic map containing 1383 DNA markers covering 1575 cM on the 12 linkage groups of rice using 186 F2 progeny from a cross between a japonica variety, ‘Nipponbare’, and an indica variety, ‘Kasalath’. Using this high-resolution molecular linkage map, we detected segregation distortion in a single wide cross of rice. The frequencies of genotypes for 1181 markers with more than 176 genotype data were plotted along this map to detect segregation distortion. Several types of distorted segregation were observed on 6 of the chromosomes. We could detect 11 major segregation distortions at ten positions on chromosomes 1, 3, 6, 8, 9, and 10. The strongest segregation distortion was at 107.2 cM on chromosome 3 and may be the gametophyte gene 2 (ga-2). The ‘Kasalath’ genotype at this position was transmitted to the progeny with about a 95% probability through the pollen gamete. At least 8 out of the 11 segregation distortions detected here are new. The use of the high-resolution molecular linkage map for improving our understanding of the genetic nature and cause of these segregation distortions is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223368
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of a YAC clone carrying the Xa-1 allele, a bacterial blight resistance gene in rice (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 117-122 
    Details
    ISSN: 1432-2242
    Keywords: Plant disease resistance ; Rice ; Xanthomonas oryzae pv. oryzae ; YAC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Map-based cloning methods have been applied for isolation of Xa-1, one of the bacterial blight resistance genes in rice.Xa-1 was previously mapped on chromosome 4 using molecular markers. For positional cloning of Xa-1, a high-resolution genetic map was made for theXa-1 region using an F2 population of 402 plants and additional molecular markers. Three restriction fragment length polymorphism (RFLP) markers, XNpb235, XNpb264 and C600 were found to be linked tightly to Xa-1, with no recombinants, and U08 750 was mapped 1.5 cM from Xa-1. The screening of a yeast artificial chromosome (YAC) library using theseXa-1-linked RFLP markers resulted in the identification of ten contiguous YAC clones. Among these, one YAC clone, designated Y5212, with an insert of 340 kb, hybridized with all three tightly linked markers. This YAC was confirmed to possess the Xa-1 allele by mapping the Xa-1 gene between both end clones of this YAC (Y5212R and Y5212L).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225736
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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