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  • 1
    Electronic Resource
    Electronic Resource
    Expression of leptin in two-layered culture of gastric mucous cells and fibroblasts: effect of Helicobacter pylori attachment (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Alimentary pharmacology & therapeutics 20 (2004), S. 0 
    Details
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background : Our recent histochemical studies have revealed an increase in myofibroblasts and in leptin and its receptor in endothelial cells, and myofibroblasts in Helicobacter pylori-infected human and Mongolian gerbil fundic mucosa.Aim : The present study was undertaken to clarify the H. pylori–induced interaction between leptin in cultured gastric surface mucous cells and fibroblasts.Methods : GSM06 cells were incubated with an air− liquid interface on a collagen gel layer containing mouse fibroblast cell line L929. Medium containing H. pylori bacilli (ATCC43504) at 10–100 times higher concentration than the GSM06 cells was added from the luminal side and the localization of leptin was observed by immunohistochemistry. The transformation of L929 cells to myofibroblasts was detected by electron microscopy and PR 2D3 immunoreactivity.Results : L929 cells in the control group showed a spindle shape with scarce cytoplasm. In the H. pylori-treated group, L929 cells showed features characteristic of myofibroblasts, and most GSM06 and L929 cells showed leptin immunoreactivity. In contrast, L929 cells incubated with H. pylori alone did not undergo this differentiation.Conclusions : Attachment of H. pylori to surface epithelial cells caused conversion of fibroblasts to myofibroblasts. We suggest that leptin plays a role in this transformation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2036.2004.01981.x
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  • 2
    Electronic Resource
    Electronic Resource
    Vital role of the itch–scratch response in development of spontaneous dermatitis in NC/Nga mice (2004)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 151 (2004), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  The itch sensation and the resultant response, scratching, are important symptoms of atopic dermatitis (AD) and have a significant impact on the quality of life of affected patients. However, the influence of the itch–scratch response on the pathology of AD has not been precisely elucidated.Objectives  To investigate the role of scratching behaviour in the development of spontaneous dermatitis using conventionally raised NC/Nga mice (Conv-NC mice), which are known to be an animal model for human AD.Methods  Capsaicin-sensitive sensory nerves of the mice were ablated by neonatal capsaicin treatment (Cap-NC mice), and the development of spontaneous dermatitis in the Cap-NC mice was compared chronologically with that in Conv-NC mice.Results  Scratching behaviour was almost completely prevented in Cap-NC mice raised for 84 days under conventional conditions, and the development of dermatitis and elevation of the serum IgE level were significantly suppressed. Histological analysis revealed that the numbers of infiltrating eosinophils and mast cells in the lesional skin of Cap-NC mice were lower than those in Conv-NC mice. Immunological studies showed that the capability of spleen T cells to produce both T-helper (Th) 1 (interferon-γ) and Th2 [interleukin (IL)-5 and IL-13] cytokines was diminished in Cap-NC mice. Furthermore, serum levels of IL-18 were approximately twice higher in Conv-NC mice than in Cap-NC mice.Conclusions  These observations suggest that scratching behaviour contributes to the development of dermatitis by enhancing various immunological responses in the murine AD model, implying that prevention of the itch sensation and/or itch-associated scratching behaviour is an effective treatment for AD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.2004.06036.x
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  • 3
    Electronic Resource
    Electronic Resource
    Fluorescence replication banding of frog chromosomes (1997)
    Springer
    Cellular and molecular life sciences 53 (1997), S. 73-77 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Late replication banding; DAPI; actinomycin D; frogs.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. To identify individual chromosomes of a frog karyotype by their fluorescence banding patterns, chromosomes were stained with actinomycin D and 4,6-diamidino-2-phenylindole (DAPI) after incorporation of BrdU during the late S-phase. The chromosomes of three Rana species which were selected for this study (R. ridibunda, R. lessonae and R. japonica) showed well-defined late replication bands. The fluorescence patterns obtained were the reverse of those produced by a 4Na-EDTA Giemsa-staining technique. Fluorescence patterns of the two water frog species (R. ridibunda and R. lessonae) were similar to each other, except for the different fluorescence of the centromeric heterochromatin, which gave extremely bright signals in R. ridibunda but no signal in R. lessonae. Experiments also showed differences between the fluorescence patterns of R. lessonae chromosome 13 in the Italian and Luxembourgian populations. These results sho w that the fluorescence replication banding using actinomycin D and DAPI is very effective in identifying individual frog chromosomes and detecting their structural changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00000582
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  • 4
    Electronic Resource
    Electronic Resource
    A Controlled Atmosphere Cutting Apparatus for Understanding Tribological Behavior of Lubricants in Near-Dry Machining (2004)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 257-258 (Feb. 2004), p. 9-14 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/47/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.257-258.9.pdf
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  • 5
    Electronic Resource
    Electronic Resource
    A possibility for new evaluating method of cytotoxicity by using heat shock protein assay (1997)
    Springer
    Journal of materials science 8 (1997), S. 143-147 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract To determine whether heat shock proteins can be utilized as a biomarker for cytotoxicity of dental materials the induction of synthesis of heat shock proteins by mercuric chloride was examined. To analyse the synthesis of heat shock proteins, HeLa cells were labelled with [35S] methionine, and the labelled proteins were separated by SDS-PAGE and autoradiographed. Incubation of the cells in a medium containing 1.25 to 40 μM mercuric chloride markedly increased the synthesis of HSP70. At 20 or 40 μM mercuric chloride in medium, HeLa cells synthesized HSP70 at 2 h after exposure, maximally at 4–7 h, and gradually diminished thereafter. Examination of the cytotoxicity of mercuric chloride by the conventional neutral red uptake assay revealed a reduction of uptake of the dye in the presence of mercuric chloride at 40 μM and above. These findings suggest that the induction of synthesis of HSP70 is one of the most sensitive cellular responses caused by mercury ion, and the heat shock protein assay can be utilized for evaluation of the cytotoxicity of dental materials.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018519102758
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  • 6
    Electronic Resource
    Electronic Resource
    Preparation and characterization of glass composite using metal particles coated with semiconductive SnO2 fine particles obtained via sol—gel method (1997)
    Springer
    Journal of materials science 8 (1997), S. 179-188 
    Details
    ISSN: 1573-482X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract SnO2—glass composites are promising materials for nitrogen-fireable thick film resistor systems. However, the SnO2—glass composite has many undesirable properties which should be improved for industrial application, such as a high electrical resistivity and a large negative temperature coefficient of electrical resistance. This work was undertaken to make a survey of the methods for improvement in the electrical properties of the SnO2—glass composites. The effect of the addition of Cu particles, with a large positive temperature coefficient of electrical resistance, on the electrical properties of SnO2—glass composites has been investigated. Cu particles have been coated with semiconductive SnO2 fine particles by hydrolysing tin and antimony ethoxides then firing. The coated particles have been applied as conductive components in the glass composite. Using SnO2-coated Cu particles, Cu and SnO2 particles are homogeneously dispersed in a glass matrix, and the electrical properties of the glass composites largely depend on the volume fraction of Cu in the glass composite. The volume fraction of Cu in the glass composite is determined by a Cu/SnO2 volume ratio in the coated particles. From the experimental evidence, it is thought feasible to produce the glass composite having well-controlled electrical properties by the suitable selection of the Cu/SnO2 ratio in the coated particles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018598214203
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  • 7
    Electronic Resource
    Electronic Resource
    Ascorbic acid prevents ischemia-reperfusion injury in the rat small intestine (1997)
    Springer
    Transplant international 10 (1997), S. 89-95 
    Details
    ISSN: 1432-2277
    Keywords: Key words Small intestine ; ascorbic acid ; reperfusion ; Ascorbic acid ; small intestine ; reperfusion ; Reperfusion ; ascorbic acid ; small intestine ; Ischemia ; ascorbic acid ; reperfusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Ischemia-reperfusion injury by free radicals and lipid peroxides is observed in various organs. Ascorbic acid (AsA) or glutathione (GSH) in various doses (AsA:2, 0.5, 0.1 mmol/kg, GSH:2 mmol/kg) was intraperitoneally administered to male Wistar rats. The entire small intestines were resected just before ischemia, after ischemia, and after 20 min of reperfusion (n = 7–10 at each time point). At each time point, the specimens were subjected to assays of lipid peroxides, GSH, and glutaminase activity of the tissues; they were also examined histologically. In the AsA group, the production of lipid peroxides after reperfusion was significantly suppressed in a dose-dependent manner, and the ratio of oxidized GSH to total GSH was also significantly low. Tissue glutaminase activity decreased to a lesser extent, and the degree of injury was apparently less marked in the AsA group. This study indicates that AsA acts as an antioxidant against peroxidative tissue injury, possibly by scavenging radicals, preserving reduced GSH, and reducing the peroxidative reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001470050018
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  • 8
    Electronic Resource
    Electronic Resource
    Anthocyanin-flavone copigmentation in bluish purple flowers of Japanese garden iris (Iris ensata Thunb.) (1997)
    Springer
    Euphytica 98 (1997), S. 163-167 
    Details
    ISSN: 1573-5060
    Keywords: Iris ensata ; copigmentation ; bluing effect ; flower color breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The in vitro identification of copigmentation was carried out using anthocyanins, such as malvidin 3RGac5G, petunidin 3RGac5G and delphinidin 3RGac5G and the flavone isovitexin. These are major pigments of Iris ensata. These anthocyanins brought about copigmentation, i.e., the bathochromic shift (bluing effect) of visible λ max due to increased concentrations of isovitexin, and 32 to 35 nm were estimated as the magnitude (Δλ max) of each shift. In addition, the absorption spectrum of 0.1 mM malvidin 3RGac5G, 0.07 mM petunidin 3RGac5G and 0.7 mM isovitexin mixture closely matched those of the fresh outer perianths of the bluish purple cultivars, ‘Suiten-isshoku’, ‘Hekikai’ and ‘Yakonotama’ which belong to the malvidin 3RGac5G - petunidin 3RGac5G type of I. ensata. Therefore, these results indicated that the bluing effect on the flower color of the bluish purple cultivars of this species was caused at least in part by the copigmentation between these anthocyanins and the flavone isovitexin. For the copigment effects of isovitexin among malvidin 3RGac5G, petunidin 3RGac5G and delphinidin 3RGac5G, λ max and Δλ max of delphinidin 3RGac5G was slightly higher than those of malvidin 3RGac5G or petunidin 3RGac5G, and the copigmentation of delphinidin 3RGac5G was characterized by a higher concentration of isovitexin. Finally, the breeding for blue flowers due to copigmentation of delphinidin 3RGac5G with isovitexin in I. ensata was discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003152813333
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of molecular weight on the production of multi-hollow polymer particles by the alkalîcooling method (1997)
    Springer
    Colloid & polymer science 275 (1997), S. 82-85 
    Details
    ISSN: 1435-1536
    Keywords: Key words Multi-hollow particle ; emulsion polymerization ; morphology ; carboxyl group ; alkali swelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  The effects of molecular weight, particle diameter and cooling condition on the formation of multi-hollow structure formed within submicron-sized styrene-methacrylic acid copolymer particles by the “alkali/cooling method” proposed by the authors were examined and the formation mechanism was proposed. The original particles were produced by emulsion copolymerization in the presence of n-octyl mercaptan as a chain transfer agent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003960050055
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  • 10
    Electronic Resource
    Electronic Resource
    Differentiation and development of Leydig cell, and induction of spermatogenesis during testicular differentiation in the Japanese eel, Anguilla japonica (1997)
    Springer
    Fish physiology and biochemistry 16 (1997), S. 187-195 
    Details
    ISSN: 1573-5168
    Keywords: gonad ; testicular differentiation ; Leydig cell ; Sertoli cell ; human chorionic gonadotropin ; spermatogenesis ; 11-ketotestosterone ; eel ; steroid producing cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The initial appearance and the development of Leydig cells (LCs), the sites of steroid hormone production in the testis, were investigated ultrastructurally during testicular differentiation in the Japanese eel, Anguilla japonica. In addition, the effects of a single injection of human chorionic gonadotropin (HCG; 5 IU g body weight-1) on histological changes of the testes and serum 11-ketotestosterone (11-KT) were examined at various stages (15–18, 20–23, 26–29, 32–35, 38–41 and 46–50 cm body length (BL)) of testicular differentiation. Testicular differentiation was morphologically characterized by the development of loose connective tissue on the medial side in animals 18–29 cm in BL. Ultrastructurally, LCs were first identified in the loose connective tissue of the testis of the 23 cm fish. In the testes of fish over 32 cm, clusters of LCs were distributed throughout the interstitial region accompanying the increase in number of spermatogonia. In fish larger than 32 cm, spermatogenesis was induced by administration of HCG; serum 11-KT levels were also raised. On the other hand, there was no effect on spermatogenesis or serum 11-KT levels in fish less than 29 cm, or in the controls. These result suggests that morphological differentiation of LCs occurs in testis of the 23 cm eel, and subsequently, the testes of eels of BL more than 32 cm acquire the capability to produce steroid hormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007753115397
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