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  • 11
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Immunological reviews 155 (1997), S. 0 
    ISSN: 1600-065X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary: NK cells express a superfamily of surface proteins that share a common structure: dimeric type II integral membrane proteins whose extracellular domains have structural features of C-type (calcium-dependent) lectins. These receptors are encoded in a single genetic region called the NK complex (NKC). The NKC encompasses several families of genes, including Ly-49 (in mice and rats). NKR-Pl (in mice, rats, and humans). NKG2 (in humans and rats), and CD94 (in humans). Different NKC receptors have been shown to activate or to inhibit NK function, and different receptors within the same family can have opposing functions. In this review, we discuss the molecular pathways by which NK cells are activated, and the mechanisms by which inhibitory receptors interrupt activation. By studying the inhibitory receptor Ly-49A, we have demonstrated that inhibition utilizes the cytoplasmic phosphatase. SHP-1, which binds to a motif in the receptor cytoplasmic domain, termed an immunoreceptor tyrosine-based inhibitory motif (ITIM). In this regard, the lectin-like receptors are functionally similar to the immunoglobulin-like killer inhibitory receptors (KIRs) on human NK cells. The presence of an ITIM generally correlates with inhibitory activity among NKC lectin-like receptors, as demonstrated by the human NKG2 receptor family Lanier and his colleagues have recently shown that NKG2 receptors can form heterodimers with the invariant lectin-like receptor CD94. Selective association of CD94 with different NKG2 receptors may explain functional differences for CD94 in different NK clones.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 23 (1997), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: We have previously reported the nucleotide sequence of the first six genes of the Streptococcuspneumoniae type 19F capsular polysaccharide biosynthesis locus (cps19f). In this study we used plasmid insertion/rescue and inverse polymerase chain reaction (PCR) to clone an additional 10 kb downstream region containing the remainder of the cps19f locus, which was then subjected to sequence analysis. The cps19f locus is located in the S. pneumoniae chromosome between dexB and aliA, and consists of 15 open reading frames (ORFs), designated cps19fA to cps19fO, that appear to be arranged as a single transcriptional unit. Insertion-duplication mutants in seven out of the nine new ORFs have been constructed in a smooth type 19F strain, all of which resulted in a rough (non-encapsulated) phenotype, confirming that the operon is essential for capsule production. Comparison with sequence databases has allowed us to propose functions for 12 of the cps19f gene products, and a biosynthetic pathway for type 19F capsular polysaccharide. T7 expression studies confirmed that cps19fH, cps19fK, cps19fL, cps19fM and cps19fN directed the production of polypeptides of the expected size in Escherichia coli. The function of the cps19fK product was confirmed by its ability to complement a mutation in nfrC (rffE ) in E. coli, as judged by restoration of sensitivity to bacteriophage N4. Interestingly, the last four genes of the locus (cps19fL–O ) exhibit very strong homology (up to 70% amino acid identity) to a portion of the Shigella flexneri rfb gene cluster encoding biosynthesis of dTDP-rhamnose. When expressed in E. coli, cps19fL–O were capable of complementing a mutation deleting the respective Shigella flexneri homologues. Southern hybridization analysis indicated that cps19fA and cps19fB were the only cps genes found in all 16 S. pneumoniae serotypes/groups tested. The region from cps19fG to cps19fK was found only in members of serogroup 19, and, within this, cps19fI was unique to type 19F.
    Type of Medium: Electronic Resource
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  • 13
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 25 (1997), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Bacteriophage λ repressor activates transcription from PRM by contacting the σ subunit of E. coli RNA polymerase. Although mutations in repressors that are defective in activation affect exposed residues in the repressor–operator co-crystal, the subunit in repressor dimers that is responsible for activation has not been determined experimentally. Here, we describe an oriented heterodimer approach using one-armed repressor–leucine zipper fusion proteins to resolve this question. Protection against methylation of operator DNA in vitro by one-armed and two-armed repressor–leucine zipper heterodimers shows that the subunit containing the arm in a one-armed repressor heterodimer binds preferentially in the consensus half-site of λ operators. The patterns of activation of the PRM promoter in vivo by one-armed heterodimers containing the pc2 mutation in only one subunit show that RNA polymerase at PRM contacts the N-terminal domain of the promoter-proximal subunit of the repressor dimer.
    Type of Medium: Electronic Resource
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  • 14
    ISSN: 1600-0668
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Medicine
    Notes: Abstract To estimate the perceptual, psychophysiological and cognitive impact of environmental tobacco smoke (ETS) on non-smokers, seventeen male never-smokers, ages 21–33, were exposed to five concentrations yielding mean ETS-respirable suspended particles (RSP) levels of 58, 113, 217, 368, and 765 μg/m3. During each 90-minute session, four smokers were seated behind a partition and smoked, when cued, to generate 70-minute exposures. For control exposures, smokers “puffed” on unlit cigarettes. Odor Strength, Annoyance, Overall Acceptance and Eye Irritation at the lowest level were significantly different from control values and the degree of change generally increased monotonically with ETS level. Fatigue was not affected by any ETS level. Odor Strength (rated highest of all attributes at all levels) at the 217 μg/m3 ETS-RSP level was 12.5% of the maximum odor intensity experienced prior to the study. No effect of ETS on information processing was observed. Psychological state and eye blink rate were affected at only the 765 μg/m3 ETS-RSP level. During times when the participants were not completing a questionnaire or test, all ETS levels resulted in a 5–8% decrease in respiratory rate, due largely to an increase in expiratory duration, but no change in minute ventilation. The breathing changes may represent a psychophysiological response mediated by the olfactory system. Non-smokers are aware of ETS at ETS-RSP concentrations as low as 58 μg/m3 (˜80-fold higher than the level typical of current U.S. workplace environments where smoking is permitted) but its sensory impact remains relatively small until ETS-RSP concentrations above 217 μg/m3 are encountered.
    Type of Medium: Electronic Resource
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  • 15
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    Leadership & organization development journal 18 (1997), S. 108-115 
    ISSN: 0143-7739
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: Investigates the issue of succession planning and implementation for chief executive officers (CEOs) at philanthropic organizations. Provides a description, definition and classification for philanthropic organizations: family, operating, community, and company-sponsored. Selects four foundation CEOs representing some of the four types noted in the typology for research. Gives them the same case study to review, read, and respond to five questions targeted to the case study and to their foundation's philosophy of succession planning. Discusses unique features and uncovers similar features and analyses reactions. Suggests that no foundation CEO interviewees had experience with succession planning or felt the choices in the study appropriate. Also identifies the need to study the process of grant awarding to uncover additional aspects related to understanding power, leadership, and influence in foundations' policies for choosing leadership and transferring authority in a planned way.
    Type of Medium: Electronic Resource
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  • 16
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature medicine 3 (1997), S. 583-585 
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Increasing use of monoclonal antibodies and recombinant proteins has created a demand for methods that accelerate and simplify generation of stable cell lines. Mammalian cell-line development involves isolation or engineering of protein-secreting cells and screening for specificity and high ...
    Type of Medium: Electronic Resource
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  • 17
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature medicine 3 (1997), S. 603-604 
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] CHRONIC OBSTRUCTIVE LUNG disease (COPD) is currently ranked twelfth among conditions that have a major impact on the global burden of disease and is predicted to rank fifth by the year 2020 (ref. 1). Pulmonary emphysema is a significant component of COPD and the destruction of the lung surface ...
    Type of Medium: Electronic Resource
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  • 18
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Peroxisomes are ubiquitous components of eukaryotic cells and are involved in numerous metabolic processes'. Newly synthesized peroxisomal proteins carry a peroxisomal targetting signal (PTS)—either PTS1 (ref. 2) or PTS2 (ref. 3) for matrix enzymes or mPTS for membrane proteins4—and are ...
    Type of Medium: Electronic Resource
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  • 19
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature genetics 17 (1997), S. 185-189 
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] In an effort to better understand the metabolic roles of the per-oxisome in both higher and lower eukaryotes, we are utilizing in silico strategies to identify novel peroxisome-associated proteins. One approach is to scan genomic and EST databases for genes capable of encoding proteins with ...
    Type of Medium: Electronic Resource
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  • 20
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Pacing and clinical electrophysiology 20 (1997), S. 0 
    ISSN: 1540-8159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Infant VT can be a devastating arrhythmia, with high mortality for those presenting with myocarditis, long QT syndrome, or cardiovascular collapse with rapid VT due to tumors. While management of these patients can be challenging and discouraging, other infants with wide QRS rhythms tend to follow a more benign course. These latter patients have accelerated idio-pathic ventricular rhythm or aberrant forms of infant supraventricular tachycardia. Distinguishing these forms of wide QRS tachycardia from the more lethal forms is paramount to institution of appropriate therapies.
    Type of Medium: Electronic Resource
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