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  • 1995-1999  (3)
  • 1915-1919
  • 1880-1889
  • 1997  (3)
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  • 1995-1999  (3)
  • 1915-1919
  • 1880-1889
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  • 1
    Electronic Resource
    Electronic Resource
    Laboratory-scale permeabilization of Escherichia coli cells for recovery of a small recombinant protein – Staphylokinase (1997)
    Springer
    Bioprocess engineering 17 (1997), S. 35-38 
    Details
    ISSN: 0178-515X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The recovery of recombinant proteins includes a purification process that has to be compressed to a minimum of steps in order to get high yields with a low cost expenditure. A selective liberation of recombinant proteins by cell permeabilization leads to both a high product purity just in the beginning of the recovery process and to a simplification of the cell residue separation compared to the mechanical cell disruption. In case of the purification of the bacterial plasminogen activator Staphylokinase from E. coli cells, yields of 82% with a purity of 46% were attained by utilization of permeabilization by biomass freezing, resuspension in a Tris/EDTA-buffer and following micro-diafiltration. A recovery process without interruption (freezing) is possible due to the addition of guanidine-HCl and Triton X100 to the buffer. These methods were developed on a laboratory-scale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008954
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Mensch und Weichtier. Umweltwissenschaften und Umweltmanagement. Von Timothy O'Riorden, Springer Verlag Berlin Heildelberg 1996, 626 Seiten, 157 Abbildungen, Übersetzung von Armin Stasch, DM 98-ISBN 3-540-61210-6 (1997)
    Weinheim : Wiley-Blackwell
    Biologie in unserer Zeit 27 (1997), S. 304-304 
    Details
    ISSN: 0045-205X
    Keywords: Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/biuz.960270512
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Die Schnecken- und Muschelfauna als Objekt der Landeskunde (1997)
    Weinheim : Wiley-Blackwell
    Biologie in unserer Zeit 27 (1997), S. 322-329 
    Details
    ISSN: 0045-205X
    Keywords: Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Bedingt durch ihre Biologie, insbesondere jedoch durch ihre enge Bindung an sehr differenzierte Habitate, stellen die Binnenmollusken für landeskulturelle Aussagen eine herausragende Tiergruppe dar. Ihre relativ geringe Ortsbeweglichkeit bei teilweise artspezifisch bedingter erheblicher passiver Verbreitungstendenz ermöglicht es ihnen, in vergleichsweise kurzer Zeit zusagende Lebensräume erfolgreich zu besiedeln. Darüber hinaus erlauben die in subfossilen Ablagerungen vielfach gut erhaltenen Gehäusereste Rückschlüsse zum historischen Faunenwandel. Ausgehend von den paläoökologischen Aussagen, die aus dem Nachweis der einzelnen Arten abgeleitet werden können, ist somit die Rekonstruktion des Landschaftsbildes zu einem konkreten Zeitpunkt möglich. Dies ist besonders wesentlich, um zwischen natürlichen Gegebenheiten und den Veränderungen, die der Mensch bedingt hat, zu unterscheiden.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/biuz.960270516
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    Paper (German National Licenses)
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