ISSN:
1432-203X
Keywords:
Key words Mitotic cyclin
;
In situ hybridization
;
Cell cycle
;
Tobacco
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have previously isolated three cDNA clones (Ntcyc25, Ntcyc27, Ntcyc29) encoding mitotic cyclins from tobacco (Nicotiana tabacum), and in this report we describe the expression patterns of these genes. RNA gel-blot analysis showed that the mitotic cyclin genes were expressed predominantly in actively dividing meristematic tissues such as the shoot apex and young developing leaves. In situ hybridization analysis indicated that while the Ntcyc29 gene was expressed consistently at low levels in shoot apex, but its transcripts were found at relatively high levels in the flower bud, indicating the possibility that the Ntcyc29 product plays a role in flower development. The Ntcyc25 and Ntcyc29 genes were expressed differentially in the root apex during the cell cycle, confirming the result obtained using synchronized-cultured tobacco cells. These results suggest that the transcriptionally regulated cyclin genes may be important in controlling cell division in tobacco.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01146775
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