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  • 1995-1999  (5)
  • 1997  (5)
  • 1
    Electronic Resource
    Electronic Resource
    FISH Mapping of Bovine U21, U1 and U7 Molecular Markers to River Buffalo Chromosomes 3p, 5q and 5p (1997)
    Springer
    Chromosome research 5 (1997), S. 337-340 
    Details
    ISSN: 1573-6849
    Keywords: cattle ; chromosome ; cosmid ; fluorescence in situ hybridization ; river buffalo ; synteny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three bovine cosmid-derived microsatellites (IDV-GA49, IDVGA7 and IDVGA47), previously assigned to cattle syntenic groups U1, U7 and U21, respectively, were fluorescence in situ hybridization (FISH) mapped to river buffalo (Bubalus bubalis, L., 2n= 50) chromosomes (BBU) 3p22 (IDVGA47, U21), BBU 5q21 (IDVGA49, U1) and BBU 5p19 (IDVGA7, U7) using sequential FISH and R-banding techniques. These localizations allowed the assignment, for the first time, of the bovine syntenic groups U21, U1 and U7 to specific river buffalo chromosomes. FISH mapping of IDVGA7 (U7) to cattle rob(1;29) p-arms confirms the banding homologies between BTA 29 and BBU 5p and further supports the idea that cattle standard karyotypes need adjustments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/B:CHRO.0000038765.65580.22
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Six bovine cosmid-derived microsatellites mapping different syntenic groups are fluorescence in situ hybridization mapped to six river buffalo chromosomes (1997)
    Springer
    Chromosome research 5 (1997), S. 541-543 
    Details
    ISSN: 1573-6849
    Keywords: cosmid ; fluorescence in situhybridization ; nomenclature ; R-banding ; river buffalo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Six bovine cosmid-derived microsatellites (IDVGA53, BTA3/U6; IDVGA61, U13; IDVGA41, BTA12/U27; IDVGA32, BTA15/U19; IDVGA59, BTA26/U26 and IDVGA71, U8), previously assigned to cattle chromosomes, were FISH-mapped to river buffalo chromosomes (BBU) 6q15, 8q34, 13q15, 16q25, 23q22 and 24q13 respectively. Sequential FISH/RBA-banding allowed the precise identification of chromosomes and localization of probe-signals on chromosome bands. These localizations allowed us to assign indirectly, for the first time, six bovine syntenic groups to river buffalo chromosomes, thereby extending its physical map. The localization of IDVGA71 (bovine U8) to the marker BBU24 adds further information to resolve definitively cattle chromosome ambiguities involving cattle chromosomes 25, 27 and 29.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018441703221
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cosmid-derived markers anchoring the bovine genetic map to the physical map (1997)
    Springer
    Mammalian genome 8 (1997), S. 29 -36 
    Details
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The mapping strategy for the bovine genome described in this paper uses large insert clones as a tool for physical mapping and as a source of highly polymorphic microsatellites for genetic typing, and was one objective of the BovMap Project funded by the European Union (UE). Eight-three cosmid and phage clones were characterized and used to physically anchor the linkage groups defining all the bovine autosomes and the X Chromosome (Chr). By combining physical and genetic mapping, clones described in this paper have led to the identification of the linkage groups corresponding to Chr 9, 12, 16, and 25. In addition, anchored loci from this study were used to orient the linkage groups corresponding to Chr 3, 7, 8, 9, 13, 16, 18, 19, and 28 as identified in previously published maps. Comparison of the estimated size of the physical and linkage maps suggests that the genetic length of the bovine genome may be around 4000 cM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003359900341
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    A medium-density genetic linkage map of the bovine genome (1997)
    Springer
    Mammalian genome 8 (1997), S. 21 -28 
    Details
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. A cattle genetic linkage map was constructed which covers more than 95 percent of the bovine genome at medium density. Seven hundred and forty six DNA polymorphisms were genotyped in cattle families which comprise 347 individuals in full sibling pedigrees. Seven hundred and three of the loci are linked to at least one other locus. All linkage groups are assigned to chromosomes, and all are orientated with regards to the centromere. There is little overall difference in the lengths of the bull and cow linkage maps although there are individual differences between maps of chromosomes. One hundred and sixty polymorphisms are in or near genes, and the resultant genome-wide comparative analyses indicate that while there is greater conservation of synteny between cattle and humans compared with mice, the conservation of gene order between cattle and humans is much less than would be expected from the conservation of synteny. This map provides a basis for high-resolution mapping of the bovine genome with physical resources such as Yeast and Bacterial Artificial Chromosomes as well as providing the underpinning for the interpolation of information from the Human Genome Project.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003359900340
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation of coding sequences from bovine cosmids by means of exon trapping (1997)
    Springer
    Mammalian genome 8 (1997), S. 486 -490 
    Details
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Exon trapping was employed to identify coding sequences from a collection of 46 bovine cosmids, previously characterized for the presence of microsatellite markers and physically mapped to chromosomes by FISH. The sequence analysis of 104 clones revealed 18 putative exons, 10 of which showed near identity to known sequences. Among these were the human (cytosine-5)-methyltransferase (DNMT), ATP-citrate lyase (ACLY), the mouse Lbcl1 oncogene, the bovine mitochondrial aconitase (ACO2) and β-arrestin 1 (ARR1). The chromosomal localization of the cloned exons was inferred from the localization of the parent cosmids. DNMT and ACLY were not previously known in cattle, but the physical localization of the cloned bovine exons is in agreement with the published comparative human and bovine maps. The trapping of exons for bovine ACO2 and ARR1 confirms the available mapping information based on synteny and provides a physical assignment for the genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003359900481
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    Paper (German National Licenses)
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