ISSN:
1573-5001
Keywords:
assignment
;
fully 13C/15N-labelled biomolecules
;
heteronuclear correlation
;
homonuclear decoupling
;
resolution enhancement
;
solid state NMR
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract High-resolution heteronuclear NMR correlation experiments and strategies are proposed for the assignment of fully13 C/15N-labelled polypeptides in the solid state. By the combination of intra-residue and inter-residue13 C-15N correlation experiments with13 C-13C spin-diffusion studies, it becomes feasible to partially assign backbone and side-chain resonances in solid proteins. The performance of sequences using 15N instead of13 C detection is evaluated regarding sensitivity and resolution for a labelled dipeptide (L-Val-L-Phe). The techniques are used for a partial assignment of the 15N and 13C resonances in human ubiquitin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008280716360
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