ISSN:
1573-6849
Keywords:
Allium
;
chiasmata
;
crossing over
;
genomic in situ hybridization
;
recombination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Genomic in situ hybridization (GISH) successfully differentiated homoeologous genomes in the interspecific hybrid Allium cepa × fistulosum, thus allowing the detection of reciprocal crossover events as label exchanges in separating anaphase I chromosomes. Three of the eight chromosome pairs were positively identified by fluorescence in situ hybridization (FISH) to rDNA sequences. There was a general similarity of the GISH-based label exchange frequencies and metaphase I chiasma frequencies, but with a 20% deficit of chiasmata. Reasons for this apparent deficit are discussed. The locations of chiasmata and label exchanges are in broad agreement.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1009296826942
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