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Genomic DNA fingerprinting of clinical isolates of Helicobacter pylori by REP-PCR and restriction fragment end-labelling (1998)

Doorn, Nathalie E.M ; Namavar, Ferry ; Kusters, Johannes G ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 160 (1998), S. 0

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ISSN: 1574-6968

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Genetic diversity of 32 Helicobacter pylori strains isolated from patients with gastritis, gastric or duodenal ulcer, carcinoma, or lymphoma was determined by repetitive sequence element polymerase chain reaction (REP-PCR), and by the new typing method restriction fragment end-labelling (RFEL). Furthermore, these two methods were used to investigate a possible correlation between clinical symptoms and the genetic background of Helicobacter pylori. Both REP-PCR and RFEL revealed 31 different patterns for the 32 strains tested, but the pair of isolates with identical REP-PCR patterns was not the same as the pair of isolates with identical RFEL patterns. Computer-assisted analysis of the DNA fingerprints was used to determine similarity coefficients. This analysis revealed no clustering of disease-specific strains by any of the two methods.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb12904.x

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Malignant fibrous histiocytoma of bone: conventional X-ray and MR imaging features (1998)

Link, T. M. ; Haeussler, M. D. ; Poppek, Susanne ; [et al.]

Springer

Skeletal radiology 27 (1998), S. 552-558

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ISSN: 1432-2161

Keywords: Key words Malignant fibrous histiocytoma ; Bone tumors ; MRI ; Conventional radiography

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Objective. To evaluate the conventional X-ray and MR imaging features of malignant fibrous histiocytoma (MFH) of bone. Design. MRI examinations and conventional radiographs were reviewed in 39 patients with biopsy-proven MFH. Imaging characteristics were analyzed and the differential diagnoses assessed in a masked fashion by two experienced radiologists. Results. Typical X-ray features included aggressive, destructive tumor growth centrally located in the metaphysis of long bones. Periosteal reactions and expansive growth were rarely seen. On MR images extraosseous tumor spread was frequently noted. On T2-weighted images and contrast-enhanced T1-weighted images most of the tumors displayed an inhomogeneous, nodular signal pattern with peripheral Gd-DTPA enhancement. Conclusions. Although several MR imaging criteria were typical for MFH none of them was specific. X-ray diagnosis of MFH may also prove difficult, with the main differential diagnosis being metastasis in the older and osteosarcoma in the younger population.