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  • 1
    ISSN: 1600-065X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary: In this article, we describe several novel genetic vaccination strategies designed to facilitate the development of different types of immune responses. These include: the consecutive use of DNA and fowlpoxvirus vectors in “prime-boost” strategies which induce greatly enhanced and sustained levels of both cell-mediated immunity and humoral immunity, including mucosal responses; ii) the co-expression of genes encoding cytokines and cell-surface receptors, and the use of immunogenic carrier molecules, for immune modulation and/or Improved targeting of vector-expressed vaccine antigens; acid iii) the expression of minimal immunogenic arnino acid sequences, particularly cytotoxic CD8+ T-cell determinants, in “polytope” vector vaccines. The capacity to modulate and enhance specific immune responses by the use of approaches such as these may underpin the development of vaccines against diseases for which no effective strategies are currently available.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford UK : Blackwell Science Ltd
    Journal of neurochemistry 72 (1999), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Single injections of cocaine, amphetamine, or methamphetamine increased RGS2 mRNA levels in rat striatum by two- to fourfold. The D1 dopamine receptor-selective antagonist SCH-23390 had no effect by itself but strongly attenuated RGS2 mRNA induction by amphetamine. In contrast, the D2 receptor-selective antagonist raclopride induced RGS2 mRNA when administered alone and greatly enhanced stimulation by amphetamine. To examine the effects of repeated amphetamine on RGS2 expression, rats were treated with escalating doses of amphetamine (1.0-7.5 mg/kg) for 4 days, followed by 8 days of multiple daily injections (7.5 mg/kg/2 h × four injections). Twenty hours after the last injection the animals were challenged with amphetamine (7.5 mg/kg) or vehicle and killed 1 h later. In drug-naive animals, acute amphetamine induced the expression of RGS2, 3, and 5 and the immediate early genes c-fos and zif/268. RGS4 mRNA levels were not affected. Prior repeated treatment with amphetamine strongly suppressed induction of immediate early genes and RGS5 to a challenge dose of amphetamine. In sharp contrast, prior exposure to amphetamine did not reduce the induction of RGS2 and RGS3 mRNAs to a challenge dose of amphetamine, indicating that control of these genes is resistant to amphetamine-induced tolerance. These data establish a role for dopamine receptors in the regulation of RGS2 expression and suggest that RGS2 and 3 might mediate some aspects of amphetamine-induced tolerance.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Westerville, Ohio : American Ceramics Society
    Journal of the American Ceramic Society 82 (1999), S. 0 
    ISSN: 1551-2916
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Cracking of granular ceramic films during drying is a well-known phenomenon. Previous work has shown that films cast above a critical thickness on nonporous substrates crack spontaneously. Films cast on previously cast layers crack at thicknesses that depend on the solids content of the slurry. Cracking occurs in this instance when films are cast above a critical saturation thickness (CST). The CST is defined as the thickness of granular material that would be 100% saturated by the liquid contained in the last cast layer. The CST is remarkably similar in magnitude to the critical cracking thickness obtained with similarly prepared slurries on dense substrates. For example, 0.5 µm Al2O3 slurries in water have a CST of 65 µm when cast on previously deposited layers of Al2O3. Similarly prepared slurries crack spontaneously when cast on single-crystal silicon at any thickness above ∼60 µm.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food process engineering 22 (1999), S. 0 
    ISSN: 1745-4530
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A study was conducted to investigate the effect of temperature on viscosity of sweet potato (SP) puree. Rheological data of the puree were measured using a Bohlin VOR Rheometer. Shear rates employed ranged from 0.01–921 s−1. Thermal scans were run at 15, 25, 40, 60, 75, and 90C. The puree exhibited “shear thinning” behavior with a yield stress value of 10 Pa at each temperature tested. The characteristic of the flow behavior of the puree was determined by fitting the experimental data to Herschel-Bulkley, Casson and Modified Casson models. The model with the best fit was then used to represent the combined effect of shear rate and temperature on the apparent viscosity of SP puree in a model based upon the Modified Casson model. The model was validated using data for SP puree samples at 50C. Results showed that, within the experimental conditions used in this study, the model could adequately predict apparent viscosities of sweet potato puree processed at different temperatures.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Reproductively mature female plaice were implanted with or without 50 μg of gonadotrophin-releasing hormone analogue (GnRHa), suspended in either coconut oil or methacrylate resin. The weight of the GnRHa-treated fish increased significantly (due to hydration of the oocytes) and reached a peak between 10 and 14 days. The fish produced several batches of eggs, which were consistently bigger than those produced by control fish. Plasma concentrations of free 17β-oestradiol and glucuronidated testosterone rose briefly (4 days) in response to the GnRHa, but then fell continuously till the end of the experiment (20 days). Plasma concentrations of sulphated 5β-pregnane-3α,17,20β-triol and 5β-pregnane-3β,17,20β-triol (which are putative metabolites of 17,20β-dihydroxy-4-pregnen-3-one, the oocyte maturation-inducing steroid) increased significantly at 4 days and reached a peak between 12 and 16 days. Concentrations were still very elevated on day 20. Plasma concentrations of sulphated 3α,17,21-trihydroxy-5β-pregnan-20-one showed a slight increase on day 4 but did not change thereafter. There was a highly significant difference in the amounts of GnRHa released into the bloodstream by the two methods of administration on day 4. However, this was not matched by significant differences in the concentrations of any of the steroids.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    International journal of quality & reliability management 16 (1999), S. 13-23 
    ISSN: 0265-671X
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: Describes the success story of how Gilman, a division of Giddings & Lewis, Inc., with the help of Blackhawk Technical College, institutionalized reliability and maintainability (R&M) into its quality system and obtained ISO 9001:1994 certification and a declaration of compliance to the QS 9000 Tooling & Equipment Supplement. Looks at aspects such as defining the need for R&M and the integration of effective reliability and maintainability procedures into the quality system. Reports on a comprehensive training effort to educate the organization in R&M.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-2137
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Sorghum bicolor (L.) Moench] grain yields can be reduced if plants are exposed to freezing temperatures prior to reaching physiological maturity. Two field experiments were conducted to evaluate chemical desiccation and panicle removal as methods for simulating the loss of carbohydrate production associated with leaf death or transport tissue damage when sorghum is exposed to freezing temperatures during grain fill. In one experiment, both treatments were repeated at 3- to 4-d intervals during grain fill with a range of hybrid maturities and environments to establish a relationship between caryopsis weight or test weight with heat unit accumulation after anthesis. This relationship was best described as sigmoidal in shape. A second field experiment compared these two treatments with potted field-grown plants exposed to -2° C for 4h. Treatments were applied four different times during grain fill. Panicle removal produced caryopsis weights 5% greater than those from freeze treatments, whereas chemical desiccation resulted in caryopsis weights 13% greater than those from freeze treatments. Test weights followed similar trends. Caryopsis weights from each treatment were similar across experiments. Panicle removal accurately simulated caryopsis weight reductions caused by exposure to freezing temperatures throughout the grain-filling period. Chemical desiccation underestimated freeze damage during early grain fill but adequately simulated caryopsis reductions during late grain fill. Test weights from panicle removal treatments were similar in both experiments, while chemical desiccation was inconsistent across experiments. Test weight reductions caused by exposure to freezing temperatures were not adequately simulated by either treatment. The results of these experiments indicate that panicle removal is a more appropriate method for simulating freeze damage to maturing grain sorghum than is chemical dessication; however, chemical desiccation may be utilized if appropriate adjustments are made during early grain fill.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-2137
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Sorghum bicolor (L.) Moench] for earlier harvest to preserve seed quality and quantity. Objectives of this study were (i) to examine the desiccant activity of glufosinate [2-amino-4-(hydroxy-methylphosphinyl)butanoic acid] applied at sever rates to grain sorghum at different seed moisture levels in contrasting environments and (ii) to determine the effect of glufosinate on germination and seedling growth of the treated crop. The soil at College Station (east-central Texas), was a Ships clay (very-fine, mixed, active, thermic, Chromic Hapludert); at Mayagüez, PR, a Coto clay (very-fine, kaolinitic, isohyperthermic Typic Hapludox). Glufosinate was evaluated because of its contact herbicidal properties and limited systemic activity. In both Texas and Puerto Rico, glufosinate was applied at 0, 0.56, 1.1, or 2.2 kg ha-1 to grain sorghum. Leaves and seed were sampled at 0, 1, 2, and 3 wk after treatment (WAT) for grain and leaf moisture. Seed was collected at 2 and 3 WAT for germination and growth studies. Glufosinate at all three treatment rates dried seed of the grain sorghum hybrid Atx638/Tx2783 in Texas from 〉 350 g kg-1 grain moisture at application to a storable moisture of 〈 120 g kg-1 at 2 WAT, at which time untreated grain moisture was 150 g kg-1. Glufosinate rate did not affect grain moisture except at the high glufosinate rate at 1 WAT. Leaf moisture reduction was glufosinate rate-dependent, but all treatments significantly reduced leaf moisture content at 1 WAT. Germination and seedling growth were not affected by glufosinate treatment. Grain desiccation rate under a tropical environment (Puerto Rico) was slightly slower than in Texas, but leaf moisture reduction was rapid and reached 〈 300 g kg-1 by 1 WAT from initial leaf moisture contents of 500 or 770 g kg-1. Germination was not adversely affected by glufosinate.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 55 (1999), S. 1272-1275 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 55 (1999), S. 1885-1888 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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