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  • 1995-1999  (3)
  • 1960-1964
  • 1950-1954
  • 1999  (3)
  • 2Cys-peroxiredoxin  (1)
  • Bamboo mosaic potexvirus  (1)
  • East/Japan Sea  (1)
Materialart
Erscheinungszeitraum
  • 1995-1999  (3)
  • 1960-1964
  • 1950-1954
Jahr
  • 1999  (3)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Journal of oceanography 55 (1999), S. 257-270 
    ISSN: 1573-868X
    Schlagwort(e): Surface heat flux ; net heat flux ; Yellow Sea ; East/Japan Sea ; East China Sea ; monthly and annual means
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geologie und Paläontologie
    Notizen: Abstract Based on the twice-daily marine atmospheric variables which were derived mostly from the weather maps for 18 years period from 1978 to 1995, the surface heat flux over the East Asian marginal seas was calculated at 0.5°×0.5° grid points twice a day. The annual mean distribution of the net heat flux shows that the maximum heat loss occurs in the central part of the Yellow Sea, along the Kuroshio axis and along the west coast of the northern Japanese islands. The area off Vladivostok turned out to be a heat-losing region, however, on the average, the amount of heat loss is minimum over the study area and the estuary of the Yangtze River also appears as a region of the minimum heat loss. The seasonal variations of heat flux show that the period of heat gain is longest in the Yellow Sea, and the maximum heat gain occurs in June. The maximum heat loss occurs in January over the study area, except the Yellow Sea where the heat loss is maximum in December. The annual mean value of the net heat flux in the East/Japan Sea is −108 W/m2 which is about twice the value of Hirose et al. (1996) or about 30% higher than Kato and Asai (1983). For the Yellow Sea, it is about −89 W/m2 and it becomes −75 W/m2 in the East China Sea. This increase in values of the net heat flux comes mostly from the turbulent fluxes which are strongly dependent on the wind speed, which fluctuates largely during the winter season.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1572-994X
    Schlagwort(e): Bamboo mosaic potexvirus ; defective RNA ; RNA combination
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract A naturally occurring 1.1 kb RNA was isolated from purified virions of bamboo mosaic potexvirus isolate S (BaMV-S). This RNA is a defective RNA (D RNA) derived from a single internal deletion of the BaMV genome. A cDNA clone representing the complete nucleotide sequence of the BaMV-S D RNA was generated and its nucleotide sequence was determined. The BaMV D cDNA is 1015 nts in length [excluding the poly(A) tail] and consists of two regions corresponding to 867 nts of the 5′ terminus and 148 nts of the 3′ terminus of the BaMV genomic RNA. BaMV D cDNA contains a single open reading frame (ORF) encoding a putative 29.7 kDa protein comprised of a fusion of the first 258 amino acids of BaMV ORF 1 and the last 2 amino acids of coat protein. The coding capacity of D RNA was verified by in vitro translation of native BaMV-S D RNA and of 1.1 kb RNA transcribed in vitro from the full-length D cDNA. BaMV D RNA can be reproducibly generated by serial passages of BaMV-S in Nicotiana benthamiana and is the first D RNA in the potexvirus group shown to be generated de novo. Alignments of sequences surrounding the 5′ and 3′ junction borders of reported potexvirus D RNAs reveal a 65.2–84.6% sequence identity, suggesting that common mechanisms for viral RNA recombination are involved in the generation of potexvirus D RNAs.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1573-5028
    Schlagwort(e): 2Cys-peroxiredoxin ; Chinese cabbage ; expression ; functional characterization ; gene cloning
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A cDNA (C2C-Prx) corresponding to a 2Cys-peroxiredoxin (2Cys-Prx) was isolated from a leaf cDNA library of Chinese cabbage. The predicted amino acid sequence of C2C-Prx has 2 conserved cysteines and several peptide domains present in most of the 2Cys-Prx subfamily members. It shows the highest sequence homology to the 2Cys-Prx enzymes of spinach (88%) and Arabidopsis (86%). Southern analysis using the cDNA insert of C2C-Prx revealed that it consists of a small multigene family in Chinese cabbage genome. RNA blot analysis showed that the gene was predominantly expressed in the leaf tissue of Chinese cabbage seedlings, but the mRNA was generally expressed in most tissues of mature plant, except roots. The expression of C2C-Prx was slightly induced by treatment with H2O2 (100μM) or Fe3+/O2/DTT oxidation system, but not by ABA (50 μM) or GA3 (10 μM). The C2C-Prx is encoded as a preprotein of 273 amino acids containing a putative chloroplast-targeting signal of 65 amino acids at its N-terminus. The N-terminally truncated recombinant protein (ΔC2C-Prx) migrates as a dimer in a non-reducing SDS-polyacrylamide gel and as a monomer in a reducing condition. The ΔC2C-Prx shows no immuno cross-reactivity to antiserum of the yeast thiol-specific antioxidant protein, and vice versa. The ΔC2C-Prx prevents the inactivation of glutamine synthetase and the DNA cleavage in the metal-catalyzed oxidation system. In the yeast thioredoxin system containing thioredoxin reductase, thioredoxin, and NADPH, the ΔC2C-Prx exhibits peroxidase activity on H2O2.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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