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  • 1
    Electronic Resource
    Electronic Resource
    New York : Cambridge University Press
    Church history 68 (1999), S. 477-479 
    ISSN: 0009-6407
    Source: Cambridge Journals Digital Archives
    Topics: History , Theology and Religious Studies
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 22 (1999), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In the alpine-treeline ecotone of the Snowy Range in Wyoming, USA, microsite sky exposure of Englemann spruce (Picea englemannii) and subalpine fir (Abies lasiocarpa) seedlings (〈 5 years) was associated with the avoidance of low-nocturnal temperatures and high insolation, factors which appeared to result in low-temperature photoinhibition. In a field experiment, light-saturated photosynthesis (Asat) in current-year seedlings (newly germinated) of fir increased significantly (approximately seven-fold) in response to increased long-wave irradiance at night (warming), solar shading (approximately five-fold), and the combination of the two treatments (approximately eight-fold). Asat in current-year spruce remained unchanged in response to all treatments, but was over four-times higher than fir in control plots. These results indicated substantial low-temperature photoinhibition, and were supported by similar Asat trends in natural seedlings. Increased needle inclination and clustering in more exposed microsites for both species implicates the possible role of structural adaptations for decreased sky exposure and warmer leaf temperatures at night.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford BSL : Blackwell Science Ltd
    Clinical & experimental allergy 29 (1999), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Macmillan Magazines Ltd.
    Nature 400 (1999), S. 251-254 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Turbulence in shelf seas strongly affects the spread of pollution (such as oil spills) as well as the distribution of sediment and phytoplankton blooms. Turbulence is known to be generated intermittently close to the sea bed, but little is known of its evolution through the water column, or to ...
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Comparative clinical pathology 9 (1999), S. 132-138 
    ISSN: 1433-2981
    Keywords: Key words:Antimicrobial peptides – Bactenecins, Bovine – Immunofluorescence assay – Monoclonal anitbody – Tissue expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: Various antimicrobial cationic peptides have been isolated from the granules of bovine neutrophils. One of these peptides is only 12 amino acids in length and is called a dodecapeptide bactenecin. A monoclonal antibody to the synthetic bovine dodecapeptide bactenecin was produced, characterised and used in an immunofluorescent assay to assess the peptide’s expression. When adult bovine peripheral blood and both adult and fetal bone marrow, tracheal lining, liver, spleen and intestinal tissues were examined, the peptide was found only within neutrophils and cells within the bone marrow. These findings both confirm and supplement another report in the literature (Storici et al. 1992), however this is the first report of a bovine antimicrobial peptide detected within the bone marrow of fetal mammals. Our results also suggest the use of immunofluorescence assays to be especially well suited for the quick screening of antimicrobial peptide tissue expression.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Archiv der Mathematik 73 (1999), S. 355-365 
    ISSN: 1420-8938
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract. For a subset E of an integral domain D and an integer-valued polynomial f over D, we investigate conditions under which the subsets E and f(E) of D determine the same integer-valued polynomials on D (this is the definition of polynomial equivalence of E and f(E)). Our primary interest in this problem lies in the case where D is the ring of rational integers. Using work of McQuillan, the case where E is finite is resolved completely in Section 3. For E infinite we show in several cases that polynomial equivalence of E and f(E) implies that f is linear, but whether this is true in general for, say, $D = \Bbb Z $ is an open question.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1017
    Keywords: Key words Raman ; Surface enhanced resonance Raman scattering spectroscopy ; Flavins ; Flavocytochrome P450 BM3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Flavocytochrome P450 BM3, an FMN-deficient mutant (G570 D), the component reductase and an FAD-containing domain were studied using surface enhanced resonance Raman scattering (SERRS). They were compared to spectra obtained from the free flavins FAD and FMN. For the holoenzyme and reductase domain, FMN is displaced during SERRS analysis. However, studies with the G570 D mutant indicate that FAD is retained in its active site. Analysis of SERRS frequencies and intensities provides information on the nature of the flavin binding site and the planarity of the ring, and enables an interpretation of the hydrogen bonding environment around ring III of the isoalloxazine moiety. Hydrogen bonding is strong at N3–H, C2=O and C4=O, but weak at N5. Structural alteration of the FAD domain of P450 BM3 is caused by removal of the FMN-binding domain. Further, the hydrogen bond at N3–H is lost and that at C2=O is weakened and the isoalloxazine ring system in the FAD domain appears to adopt a more planar arrangement. Alterations in the environment of the FAD in its isolated domain are likely to relate to changes in the redox properties and suggest a close structural interplay of FAD with the FMN-binding domain in intact flavocytochrome P450 BM3.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Surgical endoscopy and other interventional techniques 13 (1999), S. 466-468 
    ISSN: 1432-2218
    Keywords: Key words: Electromyography — Ergonomics — Laparoscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: The Society of American Gastrointestinal Endoscopic Surgeons (SAGES) Task Force on Ergonomics conducted a subjective and objective assessment of ergonomic problems associated with laparoscopic instrument use. The goal was to assess the prevalence, causes, and consequences of operational difficulties associated with the use of laparoscopic instruments. Methods: A questionnaire was distributed asking respondents to rate the frequency with which they experienced pain, stiffness, or numbness in several body areas after laparoscopic operations. An ergonomics station was assembled to quantify forearm and thumb muscle workload. Processed electromyogram (EMG) signals were acquired from 27 volunteer surgeon subjects while they completed simulated surgical tasks using a hemostat and an Ethicon® laparoscopic grasper, with the aid of an endoscopic trainer and video monitoring system. Results: Of 149 surgeons responding to the questionnaire, 8% to 12% reported frequent pain in the neck and upper extremities associated with laparoscopic surgery. The ergonomics station demonstrated that the peak and total muscle effort of forearm and thumb muscles were significantly greater (p 〈 0.01) when the grasping task was performed using the laparoscopic instrument rather than the hemostat. Conclusion: These findings indicate that laparoscopic surgical technique is more taxing on the surgeon.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 262 (1999), S. 199-206 
    ISSN: 1617-4623
    Keywords: Key words Ascidian ; Mutagenesis ; Development ; Ethylnitrosourea (ENU)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ascidians have been used extensively as model animals for experimental embryology. We report here the results of a pilot study with the aim of developing genetic methods for the ascidian Ciona savignyi. The chemical mutagen N-ethyl-N-nitrosourea (ENU) was used to induce point mutations. F1 animals, produced by using sperm from ENU-treated animals to fertilize untreated eggs, were grown to reproductive age. Sperm and eggs collected from the hermaphrodite F1 adults were used to generate self-fertilized F2 broods, which were then screened for recessive, zygotically acting mutations. Animals carrying potential mutations were outcrossed to wild type to test for the heritability of the phenotypes. We report on a number of mutants isolated using this method, including several with abnormalities in tail and notochord development.
    Type of Medium: Electronic Resource
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