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  • 1995-1999  (2)
  • 1830-1839
  • 1999  (2)
  • 2Cys-peroxiredoxin  (1)
  • East/Japan Sea  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of oceanography 55 (1999), S. 257-270 
    ISSN: 1573-868X
    Keywords: Surface heat flux ; net heat flux ; Yellow Sea ; East/Japan Sea ; East China Sea ; monthly and annual means
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Based on the twice-daily marine atmospheric variables which were derived mostly from the weather maps for 18 years period from 1978 to 1995, the surface heat flux over the East Asian marginal seas was calculated at 0.5°×0.5° grid points twice a day. The annual mean distribution of the net heat flux shows that the maximum heat loss occurs in the central part of the Yellow Sea, along the Kuroshio axis and along the west coast of the northern Japanese islands. The area off Vladivostok turned out to be a heat-losing region, however, on the average, the amount of heat loss is minimum over the study area and the estuary of the Yangtze River also appears as a region of the minimum heat loss. The seasonal variations of heat flux show that the period of heat gain is longest in the Yellow Sea, and the maximum heat gain occurs in June. The maximum heat loss occurs in January over the study area, except the Yellow Sea where the heat loss is maximum in December. The annual mean value of the net heat flux in the East/Japan Sea is −108 W/m2 which is about twice the value of Hirose et al. (1996) or about 30% higher than Kato and Asai (1983). For the Yellow Sea, it is about −89 W/m2 and it becomes −75 W/m2 in the East China Sea. This increase in values of the net heat flux comes mostly from the turbulent fluxes which are strongly dependent on the wind speed, which fluctuates largely during the winter season.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5028
    Keywords: 2Cys-peroxiredoxin ; Chinese cabbage ; expression ; functional characterization ; gene cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA (C2C-Prx) corresponding to a 2Cys-peroxiredoxin (2Cys-Prx) was isolated from a leaf cDNA library of Chinese cabbage. The predicted amino acid sequence of C2C-Prx has 2 conserved cysteines and several peptide domains present in most of the 2Cys-Prx subfamily members. It shows the highest sequence homology to the 2Cys-Prx enzymes of spinach (88%) and Arabidopsis (86%). Southern analysis using the cDNA insert of C2C-Prx revealed that it consists of a small multigene family in Chinese cabbage genome. RNA blot analysis showed that the gene was predominantly expressed in the leaf tissue of Chinese cabbage seedlings, but the mRNA was generally expressed in most tissues of mature plant, except roots. The expression of C2C-Prx was slightly induced by treatment with H2O2 (100μM) or Fe3+/O2/DTT oxidation system, but not by ABA (50 μM) or GA3 (10 μM). The C2C-Prx is encoded as a preprotein of 273 amino acids containing a putative chloroplast-targeting signal of 65 amino acids at its N-terminus. The N-terminally truncated recombinant protein (ΔC2C-Prx) migrates as a dimer in a non-reducing SDS-polyacrylamide gel and as a monomer in a reducing condition. The ΔC2C-Prx shows no immuno cross-reactivity to antiserum of the yeast thiol-specific antioxidant protein, and vice versa. The ΔC2C-Prx prevents the inactivation of glutamine synthetase and the DNA cleavage in the metal-catalyzed oxidation system. In the yeast thioredoxin system containing thioredoxin reductase, thioredoxin, and NADPH, the ΔC2C-Prx exhibits peroxidase activity on H2O2.
    Type of Medium: Electronic Resource
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