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  • 1995-1999  (3)
  • 1999  (3)
  • 3‐dimethy‐2‐imidazolidinone solvent system  (1)
  • Bamboo mosaic potexvirus  (1)
  • Key words: Differential display  (1)
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  • 1995-1999  (3)
Year
  • 1999  (3)
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Characterization of two fungal-elicitor-induced rice cDNAs encoding functional homologues of the rab-specific GDP-dissociation inhibitor (1999)
    Springer
    Planta 210 (1999), S. 143-149 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Differential display ; Functional analysis ; Fungal elicitor treatment ; Oryza (rab-GDIs) ; Pathogen signaling ; Rab-specific GDP dissociation inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. By using the mRNA differential display approach to isolate defense signaling genes active at the early stage of fungal infection two cDNA fragments with high sequence homology to rab-specific GDP-dissociation inhibitors (GDIs) were identified in rice (Oryza sativa L.) suspension cells. Using polymerase-chain-reaction products as probes, two full-length cDNA clones were isolated from a cDNA library of fungal-elicitor-treated rice, and designated as OsGDI1 and OsGDI2. The deduced amino acid sequences of the isolated cDNAs exhibited substantial homology to Arabidopsis rab-GDIs. Northern analysis revealed that transcripts detected with the 3′-gene-specific DNA probes accumulated to high levels within 30 min after treatment with a fungal elicitor derived from Magnaporthe grisea. The functionality of the OsGDIs was demonstrated by their ability to rescue the Sec19 mutant of Saccharomyces cerevisiae which is defective in vesicle transport. The proteins, expressed in Escherchia coli, cross-reacted with a polyclonal antibody prepared against bovine rab-GDI. Like bovine rab-GDI, the OsGDI proteins efficiently dissociated rab3A from bovine synaptic membranes. Using the two-hybrid system, it was shown that the OsGDIs specifically interact with the small GTP-binding proteins belonging to the rab subfamily. The specific interaction was also demonstrated in vitro by glutathione S-transferase resin pull-down assay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050663
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A Defective RNA Associated with Bamboo Mosaic Virus and the Possible Common Mechanisms for RNA Recombination in Potexviruses (1999)
    Springer
    Virus genes 18 (1999), S. 121-128 
    Details
    ISSN: 1572-994X
    Keywords: Bamboo mosaic potexvirus ; defective RNA ; RNA combination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A naturally occurring 1.1 kb RNA was isolated from purified virions of bamboo mosaic potexvirus isolate S (BaMV-S). This RNA is a defective RNA (D RNA) derived from a single internal deletion of the BaMV genome. A cDNA clone representing the complete nucleotide sequence of the BaMV-S D RNA was generated and its nucleotide sequence was determined. The BaMV D cDNA is 1015 nts in length [excluding the poly(A) tail] and consists of two regions corresponding to 867 nts of the 5′ terminus and 148 nts of the 3′ terminus of the BaMV genomic RNA. BaMV D cDNA contains a single open reading frame (ORF) encoding a putative 29.7 kDa protein comprised of a fusion of the first 258 amino acids of BaMV ORF 1 and the last 2 amino acids of coat protein. The coding capacity of D RNA was verified by in vitro translation of native BaMV-S D RNA and of 1.1 kb RNA transcribed in vitro from the full-length D cDNA. BaMV D RNA can be reproducibly generated by serial passages of BaMV-S in Nicotiana benthamiana and is the first D RNA in the potexvirus group shown to be generated de novo. Alignments of sequences surrounding the 5′ and 3′ junction borders of reported potexvirus D RNAs reveal a 65.2–84.6% sequence identity, suggesting that common mechanisms for viral RNA recombination are involved in the generation of potexvirus D RNAs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008008400653
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Reaction characteristics of cellulose in the LiCl/1,3‐dimethyl‐2‐imidazolidinone solvent system (1999)
    Springer
    Cellulose 6 (1999), S. 93-102 
    Details
    ISSN: 1572-882X
    Keywords: LiCl/1 ; 3‐dimethy‐2‐imidazolidinone solvent system ; homogeneous cellulose solution ; cellulose acetate ; O‐methylcellulose ; reaction characteristics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In order to elucidate the nature of the LiCl/1,3‐dimethy‐2‐imidazolidinone (DMI) solvent system as one of the homogeneous reaction media of cellulose, cellulose acetate (CA) and O‐methylcellulose (MC) were prepared using this solvent system, and the distribution of substituents within anhydroglucose units was examined by 13C‐NMR. It was found that (i) homogeneous cellulose solutions can be easily prepared by heating 2, 5–12 and 100 parts of weight of cellulose, LiCl, and DMI, respectively, and (ii) the relative reactivity of hydroxyl groups is in the order C‐6 〉 C‐2 〉 C‐3 for both CA and MC. A remarkable feature of this solvent system is that the reaction efficiency in etherification is very high compared with other homogeneous solvent systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009208417954
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    Paper (German National Licenses)
    Fulltext
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