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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 98 (1999), S. 213-218 
    ISSN: 1432-2242
    Keywords: Key words ITS sequence analysis ; Cycle sequencing ; Lilium ; Hybrid verification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Cycle sequencing of the ITS region of nuclear ribosomal DNA of L. concolor, L. dauricum and L. maculatum generated surprisingly homogenous sequences from these three species. Analysis of the few (13 out of 639) polymorphic nucleotide sites in the ITS region produced results that do not support the belief that L. maculatum is a hybrid of the two other species. Neighbor-Joining analysis of the genetic distances calculated using the Kimura 2-parameter model of base substitution confirmed the close relationship between L. dauricum and L. maculatum. The phylogenetic tree, in conjunction with the distribution pattern and morphological similarities of the two species, suggest that L. maculatum is derived from the more widely distributed L. dauricum. The results also revealed that there is sufficient molecular divergence between L. maculatum and L. dauricum to support their status as separate species.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 98 (1999), S. 954-960 
    ISSN: 1432-2242
    Keywords: Key words Lilium ; Molecular systematics ; ITS DNA sequences ; Phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The DNA from 16 Lilium species and one variety endemic to or naturalized in Japan were obtained and their internal transcribed spacer regions of nuclear ribosomal DNA (nrDNA) were amplified by PCR and sequenced by cycle sequencing. Phylogenetic analysis of the ITS sequences supported the validity of Comber’s classification system. It has also provided molecular evidence for the transfer of Lilium dauricum to sect. Sinomartagon. The phylogenetic relationships revealed by ITS DNA analysis were supported by previously published crossability data. The molecular phylogeny of Japanese Lilium species was discussed with reference to the putative migration routes of these species.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 98 (1999), S. 422-433 
    ISSN: 1432-2242
    Keywords: Key words Allium ; Nectaroscordum ; Caloscordum ; Subgenus Amerallium ; Phylogeny ; ITS sequence analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Phylogenetic analysis of ITS DNA sequences of various Old and New World Alliums suggests the reinclusion of Nectaroscordum and affirms the reestablishment of Caloscordum as subgenera of genus Allium. The results sanction the elimination of the Old World Allium species from subg. Amerallium Traub and endorse Wendelbo’s definition of subg. Molium (excluding sect. Porphyrason).
    Type of Medium: Electronic Resource
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