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  • 1
    ISSN: 1438-2385
    Keywords: Key words Continuous flow microwave treatment ; Milk ; Vitamin B1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The effects of continuous flow microwave treatment and conventional heating (using a plate heat exchanger) on the nutritional value of raw milk was estimated by measurement of the vitamin B1 retention. Microwave heating, without a holding phase, did not modify the vitamin B1 content of milk, whereas analogous treatment carried out with the plate heat exchanger led to a loss of this vitamin. A holding phase caused a similar loss of vitamin B1 in milk heated using either system. These results indicate that continuous flow microwave treatment of milk compares favourably with conventional heating because it produce less destruction of vitamin B1 in milk. This could be attributed to the shorter time taken to reach the final temperature and the lack of hot surfaces in contact with the milk in the case of the microwave system.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1438-2385
    Keywords: Key words Meat ; Thiamine ; Capillary electrophoresis ; High performance liquid chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A new capillary electrophoresis (CE) method for the analysis of thiamine in meat is proposed. Samples were submitted to acidic and enzymatic hydrolysis and the extracts were purified using ethanol and an ion exchange column. The thiamine content was determined by CE using 100 mM sodium tetraborate, 50 mM sodium phosphate (pH 7.6), 50 mM sodium dodecyl sulphate and 10% isopropyl alcohol as a separation buffer solution. The analysis was carried out at 15 kV and 50  °C in a 70 cm effective length× 75 μm i.d. fused-silica capillary using on-column UV detection at 254 nm and 7 s injection time (27 nl injection volume). The results obtained by CE for thiamine contents in meat were compared to those obtained by HPLC using an ion-pair reverse phase column with post-column derivatization and fluorescence detection.
    Type of Medium: Electronic Resource
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