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Notes: Ion distributions in a loss cone are measured in the GAMMA 10 tandem mirror [Phys. Rev. Lett. 55, 939 (1985)]. When Alfvén ion cyclotron (AIC) modes are excited in the central cell, enhanced end-loss of ions is observed for a characteristic energy region which agrees well with the resonant ion cyclotron condition with the AIC wave. From measurements of pitch angle distributions of end-loss ions, it is found that the loss cone is filled for this characteristic energy region, indicating an ion diffusion in a velocity space induced by the AIC wave. Magnitude of the observed additional end-loss increases with increase in the amplitude of the AIC modes. In the present experimental parameters in GAMMA 10, the AIC wave effect on the axial ion confinement is not serious. © 2000 American Institute of Physics.

Notes: A pressure driven m=1/n=1 mode is excited by lower hybrid current drive in the WT-3 tokamak [T. Maehara et al., Nucl. Fusion 38, 39 (1998)]. The excitation of the mode is accompanied with the decrease of the magnetic shear and with the peaking of the soft x-ray emissivity profile inside the q=1 surface. The crescent-shaped mode structure appeared on the contour map of the soft x-ray emissivity is consistent with that of the quasi-interchange mode. The m=1 mode can be suppressed by electron cyclotron heating near the q=1 surface. The range of the location of the electron cyclotron resonance layer effective for the complete suppression is much wider and the time scale for the suppression is much faster than those in the case of the suppression of the tearing mode in the ohmic heating plasma. © 2000 American Institute of Physics.

Notes: A high precision atomic polarimeter system for the use in beam-foil spectroscopy experiments with a few keV/amu heavy-ion beams has been developed. The polarimeter measures the circular polarization of fluorescences from the beam ion in-flight after the beam–foil interaction. The present system has two identical such polarimeters in both sides of the beam axis to reduce the systematic errors such due to the fluctuations of beam current, background and so on. A successful use of an ultrathin carbon foil (1.5 μg/cm2), which was durable for several hours against a few hundred nA beam irradiation, enabled the beam–foil experiments with such low energy heavy-ion beams. A performance test of the polarimeter system was carried out in the tilted foil experiments with a 1.7 keV/amu 14N+ beam. The atomic polarization was observed for the transition 1s22s22p3p 1D→1s22s22p3p 1P, whose fluorescence wavelength is 399.5 nm, in the N+ ion (N II). The polarization was approximately −2% for the tilt angle of −40° and showed monotone increasing with increasing tilt angle up to +2% for +40°. The polarization at 0° was (0.002±0.25)%, which is highly consistent with the expected polarization of 0%. This result indicates the high reliability of the present polarimeter system. This is the first tilted-foil experiment at such low beam energy. The present experimental technique will be very useful for studies of the polarization mechanism of the beam–foil interaction. © 2000 American Institute of Physics.

Notes: Background  Atopic dermatitis (AD) is a chronic and relapsing eczematous skin disorder characterized by eosinophilia. Nerve growth factor (NGF) modulates the allergic response through interactions with immune-inflammatory cells. Eosinophils have been reported to store NGF as a preformed mediator.Objective  To gain further insight into the significance of eosinophils in association with NGF in the pathogenesis of AD, the localization of NGF within eosinophils and the difference of the eosinophil-derived NGF content in the peripheral blood of normal volunteers vs. AD patients were investigated.Methods  We examined the localization of NGF within human eosinophils using the post-embedding immunoelectron microscopy and compared NGF content in freshly isolated eosinophil sonicates from the peripheral blood of 31 normal volunteers vs. 42 AD patients by immunoenzymatic assay. A possible correlation between the levels of NGF and major basic protein was also examined.Results  Immunoelectron microscopic studies revealed that NGF was localized in the central core of normal eosinophil granules, where major basic protein is also present as a preformed mediator, in homogeneous granules and in intergranular ductal or vesicular structures adjacent to specific granules of eosinophils. NGF content in eosinophils was significantly increased in AD patients. Furthermore, there was a significant correlation between levels of NGF and major basic protein in eosinophils of AD patients.Conclusions  Increased levels of NGF contained in eosinophils of the peripheral blood from AD patients, when released with other mediators such as basic proteins, could promote inflammation and local tissue damage.

Notes: The purpose of the current study was to evaluate the clinical performance of a hybrid composite material used as a prosthetic veneering agent after servicing for more than 4 years. A photo-curable composite material (Cesead®) was selected as the veneering agent. Composite resin veneered restorations made with the composite and a noble metal alloy were inserted into 110 teeth of 40 patients. Modified United States Public Health Service (USPHS) criteria were used for direct evaluation of colour matching ability, veneer–metal interfacial staining, veneer surface texture, staining of the metal casting, and wear of the veneer–metal interface. After an average observation period of 5 years and 3 months, the rate of restorations considered to be clinically ideal (Alfa) was 75·5% for colour match, 93·6% for veneer–metal interfacial staining, 82·7% for veneer surface texture, 97·3% for staining of the metal casting, and 95·5% for wear. The restorations judged as clinically unacceptable (Charlie) consisted of only one case for colour match and three cases for veneer surface texture. Although the Alfa rate decreased with increasing length of service, the Cesead® composite is considered to be a clinically reliable material as a prosthetic veneering agent.

Notes: Background : Our recent histochemical studies have revealed the marked increase of myofibroblasts in the Helicobacter pylori-infected Mongolian gerbil fundic mucosa, while the mediators, which facilitate the conversion of fibroblasts to the myofibroblasts have remained unknown.Aim : The present study was undertaken to clarify the alteration of leptin in the control and H. pylori-infected Mongolian gerbil stomach. The effector sites of rebamipide were also investigated in relation to leptin.Methods : The localization of leptin was investigated by the indirect immunofluorescence. Plasma leptin levels were determined by ELISA method. The localization of 3H-rebamipide binding sites was investigated by autoradiography.Results : Serum leptin content in H. pylori-infected Mongolian gerbils was significantly increased. The presence of leptin immunoreactivity was recognized in the endothelial cells of the microcirculatory network and very weakly in the glandular cells in the control group, while in the H. pylori-infected group leptin was markedly recognized in the mesenchymal cells. Rebamipide bound to the fibroblasts and surface mucous cells and decreased the leptin immunoreactivity in the gastric mucosa.Conclusions : Leptin was mostly found in the mesenchymal cells. Rebamipide administration brought about the decrease of leptin in the gastric mucosaof the H. pylori-infected gerbils.

Notes: Background: Monoclonal antibodies against GAP43 and synaptophysin, markers of regenerated nerves, have recently become available. Aim: To investigate the regeneration of the autonomic nerves after acetic acid treatment, as well as the effect of recombinant basic fibroblast growth factor (bFGF-CS23) and sofalcone on reinnervation. Methods: Ulcers were induced by the direct application of 100% acetic acid to the serosal surface of the rat fundic stomach. Some rats were treated with bFGF-CS23 or sofalcone every 12 h after the acetic acid treatment. The immunohistochemical location of GAP43 and synaptophysin was observed by confocal laser microscopy, and the uptake sites of 14C-sofalcone were observed by autoradiography. Results: Both GAP43 and synaptophysin immuno-reactivities surrounding microvessels were weak in the control group, whereas in the acetic acid-treated group, these immunoreactivities were increased. Treatment with bFGF-CS23 and sofalcone increased these immunoreactivities. The binding sites of sofalcone coincided with the location of regenerated nerves and surface mucous cells. The progenitors of the autonomic nerves were more abundant than expected. Conclusion: Both bFGF and sofalcone seem to stimulate nerve regeneration.

Notes: Background : Rebamipide is a gastroprotective agent to stimulate prostaglandin generation in gastric mucosa and attenuate the activity of neutrophils, but direct evidence for the effector sites of this agent has remained to be clarified.Aim : The present study was undertaken to show the effector sites of rebamipide in control and ulcer-provoked rats.Methods : The rats were divided into control, acetic acid- and ethanol-treated rats. In the acetic acid-treated group, 100% acetic acid was attached to the serosal surface of the stomach for 30 s, 7 days before the experiments. In the ethanol-treated group, a dose of 0.5 mL/100 g body weight of 50% ethanol was administered through orogastric intubation 2 h before the experiments. Using the unfixed cryostat sections, aqueous solution of 3H-rebamipide was applied and the localization of the binding sites of rebamipide was investigated by autoradiography.Results : In the control rats, rebamipide was found to bind to the surface epithelial cells. In the ethanol-treated group, few binding sites were observed in the damaged gastric mucosa. In the acetic acid-treated group, the marked accumulation of the binding sites of 3H-rebamipide was observed in the mesenchymal cells in the lamina propria mucosae between the regenerated gastric epithelial cells. Combination of autoradiography and immunohistochemistry has revealed that iNOS-immunoreactive cells had the strong binding of rebamipide in the acetic acid-treated group. Some of these cells were CD68-positive macrophages, while others were CD68-negative, corresponding to polymorphonuclear leucocytes. In the ethanol-treated acute gastric mucosal injury group, few binding sites were observed in the damaged gastric mucosa.Conclusions : Autoradiography has made it clear that rebamipide binds to iNOS-positive cells in the gastric mucosa 7 days after acetic acid-treatment.

Notes: Background : Omeprazole 10 mg is used as maintenance therapy for gastro-oesophageal reflux disease, but previous reports have not mentioned the potency of its acid suppression.Aim : To evaluate the potency of acid suppression with omeprazole 10 mg, in relation to CYP2C19 genotypes.Methods : Eighteen healthy subjects without Helicobacter pylori participated. After a 7-day regimen of omeprazole 10 mg, 20 mg, lafutidine 20 mg (a novel H2-receptor antagonist) or water only (baseline data), intragastric pH was measured for 24 h.Results : With omeprazole 10 mg, greater differences were observed than 20 mg in median pH values and pH 〉 4 holding time ratios between poor metabolizers (PMs, n = 6) and the others [homozygous extensive metabolizers (homo-EMs, n = 6) and heterozygous extensive metabolizers (hetero-EMs, n = 6)]. With lafutidine 20 mg, these parameters were not influenced by the genotype. The potency of acid suppression was: omeprazole 20 mg ≈ lafutidine 20 mg 〉 omeprazole 10 mg in homo-EMs, omeprazole 20 mg 〉 omeprazole 10 mg ≈ lafutidine 20 mg in hetero-EMs, and omeprazole 20 mg ≈ omeprazole 10 mg 〉 lafutidine 20 mg in PMs.Conclusions : Omeprazole 10 mg strongly suppresses acid secretion, but depending on the CYP2C19 genotypes shows greater interindividual variations in suppression than 20 mg.

Notes: Background  Although some patients with psoriasis vulgaris also complain of severe pruritus, the data available regarding pruritus in psoriasis are sparse.Objectives  To clarify the mechanism and mediators involved in the pruritus of psoriasis vulgaris, we compared itch-associated factors in lesional skin from psoriatic patients vs. skin without pruritus quantitatively using a panel of histological and immunohistological parameters.Patients and methods  Biopsied specimens were obtained from 38 patients with psoriasis vulgaris who were divided into two groups according to the presence or absence of pruritus.Results  When compared with psoriatic patients devoid of pruritus, lesional skin from patients with pruritus showed the following characteristic features: (i) a rich innervation both in the epidermis and in the papillary dermis; (ii) an increase in neuropeptide substance P-containing nerve fibres in perivascular areas; (iii) decreased expression of neutral endopeptidase in the epidermal basal layer as well as in the endothelia of blood vessels; (iv) many mast cells showing degranulating processes in the papillary dermis; (v) a strong immunoreactivity for nerve growth factor (NGF) throughout the entire epidermis and an increased NGF content in lesional skin homogenates; (vi) an increase in the expression of high-affinity receptors for NGF (Trk A) in basal keratinocytes and in dermal nerves; (vii) an increased population of interleukin-2-immunoreactive lymphocytes; and (viii) a strong expression of E-selectin on vascular endothelial cells. A significant correlation was observed between the severity of pruritus and protein gene product 9.5-immunoreactive intraepidermal nerve fibres, NGF-immunoreactive keratinocytes, expression of Trk A in the epidermis and the density of immunoreactive vessels for E-selectin. These findings indicate that possible pruritogenic mediators in psoriatic lesional skin are neurogenic factors including innervation, neuropeptide substance P, neuropeptide-degrading enzymes and NGF, activated mast cells, one or more cytokines and endothelial–leucocyte adhesion molecules.Conclusions  These data document for the first time itch-related local markers in psoriasis, and suggest complex and multifactorial mechanisms of pruritus in the disease. These results provide the groundwork for further studies to evaluate the efficacy of antipruritic treatment for psoriatic patients.